Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis

Claudia Fischer; Marc Drillich; Simone Odau; Wolfgang Heuwieser; Ralf Einspanier; Christoph Gabler

doi:10.1071/rd09120

journal article Apr 07, 2010

Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis

Claudia Fischer Marc Drillich Simone Odau Wolfgang Heuwieser Ralf Einspanier Christoph Gabler

Reproduction, Fertility and Development Vol. 22 No. 5 pp. 818-829 · CSIRO Publishing

View at Publisher Save 10.1071/rd09120

Abstract

Endometrial cells take part in embryo–maternal communication, as well as supporting the immune system in defending against invading pathogens. The aim of the present study was to examine the mRNA expression of factors that have been suggested to be involved in both events in the bovine endometrial epithelium, namely bovine granulocyte chemotactic protein 2 (CXCL5), interleukin-1 beta (IL1B), IL6, IL8, tumour necrosis factor (TNF), cyclooxygenase 2 (PTGS2) and haptoglobin (HP). Samples were collected in vivo from cows on Days 21–27 postpartum by the cytobrush method to evaluate the correlation between inflammatory factors and uterine health (cows with signs of clinical or subclinical endometritis and healthy cows). Bovine uteri were collected at the abattoir to investigate oestrous cycle-dependent mRNA expression patterns. Real-time reverse transcription–polymerase chain reaction revealed that the expression of CXCL5, IL1B, IL8 and TNF mRNA was significantly higher in cows with subclinical or clinical endometritis compared with healthy cows. The expression of CXCL5, IL1B and IL8 mRNA was increased around ovulation compared with the luteal phase. There was no indication of either oestrous cycle-dependent expression or a correlation with uterine health for IL6, PTGS2 and HP transcripts. These results suggest that CXCL5, IL1B, IL8 and TNF may represent potential marker genes for the detection of cows with subclinical endometritis and for monitoring new therapeutic approaches.

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References

Details

Published: Apr 07, 2010
Vol/Issue: 22(5)
Pages: 818-829
License: View

Authors

C

Claudia Fischer

AInstitute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.

M

Marc Drillich

BClinic for Animal Reproduction, Freie Universität Berlin, Königsweg 63, 14163 Berlin, Germany.

S

Simone Odau

AInstitute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.

W

Wolfgang Heuwieser

BClinic for Animal Reproduction, Freie Universität Berlin, Königsweg 63, 14163 Berlin, Germany.; CPresent address: Animal Welfare Program, Faculty of Land and Food Systems, The University of British Columbia, 2357 Main Mall, Vancouver V6T 1Z4, Canada.

R

Ralf Einspanier

AInstitute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.

C

Christoph Gabler

AInstitute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.; DCorresponding author. Email: gablerch@zedat.fu-berlin.de

Cite This Article

Claudia Fischer, Marc Drillich, Simone Odau, et al. (2010). Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis. Reproduction, Fertility and Development, 22(5), 818-829. https://doi.org/10.1071/rd09120

Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis

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