Identification of three LRR-RKs involved in perception of root meristem growth factor in
                    Arabidopsis

Hidefumi Shinohara; Ayaka Mori; Naoko Yasue; Kumiko Sumida; Yoshikatsu Matsubayashi

doi:10.1073/pnas.1522639113

journal article Mar 21, 2016

Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis

Hidefumi Shinohara Ayaka Mori Naoko Yasue Kumiko Sumida Yoshikatsu Matsubayashi

Proceedings of the National Academy of Sciences Vol. 113 No. 14 pp. 3897-3902 · Proceedings of the National Academy of Sciences

View at Publisher Save 10.1073/pnas.1522639113

Abstract

Significance
There is a growing awareness that receptor kinases (RKs) regulate various aspects of plant development in response to perception of specific ligands, but the highly redundant nature of this family's members often makes it difficult to identify their ligands by a conventional mutant screening approach. To sidestep gene redundancy problems, here we used a combination of a custom-made RK expression library and exhaustive photoaffinity labeling and identified three RKs that directly interact with root meristem growth factor (RGF), a peptide hormone regulating root meristem development. The receptor triple mutant was insensitive to externally applied RGF peptide and displayed a short root phenotype accompanied by a considerable decrease in meristematic cell number, representing a previously unidentified ligand–receptor system in plants.

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Metrics

182

Citations

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References

Details

Published: Mar 21, 2016
Vol/Issue: 113(14)
Pages: 3897-3902

Authors

H

Hidefumi Shinohara

Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan;

A

Ayaka Mori

Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan;

N

Naoko Yasue

National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan

K

Kumiko Sumida

National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan

Y

Yoshikatsu Matsubayashi

Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan;

Cite This Article

Hidefumi Shinohara, Ayaka Mori, Naoko Yasue, et al. (2016). Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis. Proceedings of the National Academy of Sciences, 113(14), 3897-3902. https://doi.org/10.1073/pnas.1522639113

Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis

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