The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules.

H I Chen; M Sudol

doi:10.1073/pnas.92.17.7819

journal article Open Access Aug 15, 1995

The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules.

Proceedings of the National Academy of Sciences Vol. 92 No. 17 pp. 7819-7823 · Proceedings of the National Academy of Sciences

View at Publisher Save 10.1073/pnas.92.17.7819

Abstract

The WW domain has previously been described as a motif of 38 semiconserved residues found in seemingly unrelated proteins, such as dystrophin, Yes-associated protein (YAP), and two transcriptional regulators, Rsp-5 and FE65. The molecular function of the WW domain has been unknown until this time. Using a functional screen of a cDNA expression library, we have identified two putative ligands of the WW domain of YAP, which we named WBP-1 and WBP-2. Peptide sequence comparison between the two partial clones revealed a homologous region consisting of a proline-rich domain followed by a tyrosine residue (with the shared sequence PPPPY), which we shall call the PY motif. Binding assays and site-specific mutagenesis have shown that the PY motif binds with relatively high affinity and specificity to the WW domain of YAP, with the preliminary consensus XPPXY being critical for binding. Herein, we have implicated the WW domain with a role in mediating protein-protein interactions, as a variant of the paradigm set by Src homology 3 domains and their proline-rich ligands.

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Published: Aug 15, 1995
Vol/Issue: 92(17)
Pages: 7819-7823

Authors

H

H I Chen

Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021, USA.

M

M Sudol

Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021, USA.

Cite This Article

H I Chen, M Sudol (1995). The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules.. Proceedings of the National Academy of Sciences, 92(17), 7819-7823. https://doi.org/10.1073/pnas.92.17.7819

The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules.

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