CD44 is a physiological E-selectin ligand on neutrophils

Yoshio Katayama; Andres Hidalgo; Jungshan Chang; Anna Peired; Paul S. Frenette

doi:10.1084/jem.20042014

journal article Apr 11, 2005

CD44 is a physiological E-selectin ligand on neutrophils

Yoshio Katayama Andres Hidalgo

Jungshan Chang

Anna Peired Paul S. Frenette

The Journal of Experimental Medicine Vol. 201 No. 8 pp. 1183-1189 · Rockefeller University Press

View at Publisher Save 10.1084/jem.20042014

Abstract

The selectin family of adhesion molecules and their glycoconjugated ligands are essential for blood polymorphonuclear neutrophil (PMN) extravasation into inflammatory and infectious sites. However, E-selectin ligands on PMNs are not well characterized. We show here that CD44 immunopurified from G-CSF–differentiated 32D cells or from peripheral blood PMNs binds specifically to E-selectin. In contrast, CD44 extracted from bone marrow stromal or brain endothelial cell lines does not interact with E-selectin, suggesting cell-specific posttranslational modifications of CD44. PMN-derived CD44 binding activity is mediated by sialylated, α(1,3) fucosylated, N-linked glycans. CD44 enables slow leukocyte rolling on E-selectin expressed on inflamed endothelium in vivo and cooperates with P-selectin glycoprotein ligand–1 to recruit neutrophils into thioglycollate-induced peritonitis and staphylococcal enterotoxin A–injected skin pouch. CD44 extracted from human PMNs also binds to E-selectin. Moreover, we demonstrate that CD44 is hypofucosylated in PMNs from a patient with leukocyte adhesion deficiency type II, suggesting that it contributes to the syndrome. These findings thus suggest broader roles for CD44 in the innate immune response and uncover a potential new target for diseases in which selectins play a prominent role.

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Details

Published: Apr 11, 2005
Vol/Issue: 201(8)
Pages: 1183-1189

Authors

Y

Yoshio Katayama