Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens

Sarah L. Jongbloed; Andrew J. Kassianos; Kylie J. McDonald; Georgina J. Clark; Xinsheng Ju; Catherine E. Angel; Chun-Jen J. Chen; P. Rod Dunbar; Robert B. Wadley; Varinder Jeet; Annelie J.E. Vulink; Derek N.J. Hart; Kristen J. Radford

doi:10.1084/jem.20092140

journal article May 17, 2010

Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens

Kylie J. McDonald Georgina J. Clark Xinsheng Ju Catherine E. Angel Chun-Jen J. Chen P. Rod Dunbar Robert B. Wadley Varinder Jeet Annelie J.E. Vulink Derek N.J. Hart Kristen J. Radford

Journal of Experimental Medicine Vol. 207 No. 6 pp. 1247-1260 · Rockefeller University Press

View at Publisher Save 10.1084/jem.20092140

Abstract

The characterization of human dendritic cell (DC) subsets is essential for the design of new vaccines. We report the first detailed functional analysis of the human CD141+ DC subset. CD141+ DCs are found in human lymph nodes, bone marrow, tonsil, and blood, and the latter proved to be the best source of highly purified cells for functional analysis. They are characterized by high expression of toll-like receptor 3, production of IL-12p70 and IFN-β, and superior capacity to induce T helper 1 cell responses, when compared with the more commonly studied CD1c+ DC subset. Polyinosine-polycytidylic acid (poly I:C)–activated CD141+ DCs have a superior capacity to cross-present soluble protein antigen (Ag) to CD8+ cytotoxic T lymphocytes than poly I:C–activated CD1c+ DCs. Importantly, CD141+ DCs, but not CD1c+ DCs, were endowed with the capacity to cross-present viral Ag after their uptake of necrotic virus-infected cells. These findings establish the CD141+ DC subset as an important functionally distinct human DC subtype with characteristics similar to those of the mouse CD8α+ DC subset. The data demonstrate a role for CD141+ DCs in the induction of cytotoxic T lymphocyte responses and suggest that they may be the most relevant targets for vaccination against cancers, viruses, and other pathogens.

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Details

Published: May 17, 2010
Vol/Issue: 207(6)
Pages: 1247-1260

Authors

S

Sarah L. Jongbloed

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1

A

Andrew J. Kassianos

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1; University of Queensland Faculty of Health Sciences, Herston, Queensland 4029, Australia 2

K

Kylie J. McDonald

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1

G

Georgina J. Clark

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1; University of Queensland Faculty of Health Sciences, Herston, Queensland 4029, Australia 2

X

Xinsheng Ju

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1

C

Catherine E. Angel

School of Biological Sciences 3 and 4; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand 3 and 4

C

Chun-Jen J. Chen

School of Biological Sciences 3 and 4; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand 3 and 4

P

P. Rod Dunbar

School of Biological Sciences 3 and 4; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand 3 and 4; School of Biological Sciences 3 and 4; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand 3 and 4

R

Robert B. Wadley

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1

V

Varinder Jeet

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1

A

Annelie J.E. Vulink

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1

D

Derek N.J. Hart

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1; University of Queensland Faculty of Health Sciences, Herston, Queensland 4029, Australia 2; Cooperative Research Centre for Biomarker Translation, La Trobe University, Bundoora, Victoria 3086, Australia 5

K

Kristen J. Radford

Dendritic Cell Program, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia 1; University of Queensland Faculty of Health Sciences, Herston, Queensland 4029, Australia 2

Cite This Article

Sarah L. Jongbloed, Andrew J. Kassianos, Kylie J. McDonald, et al. (2010). Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. Journal of Experimental Medicine, 207(6), 1247-1260. https://doi.org/10.1084/jem.20092140

Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens

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