High frequency of increased triclosan MIC among CC5 MRSA and risk of misclassification of the SCCmec into types

Alice Slotfeldt Viana; Ana Maria Nunes Botelho; Andries Feder; Ahmed Magdi Moustafa; Deborah Nascimento Santos Silva; Caroline Lopes Martini; Adriana Lúcia Pires Ferreira; Maria Cícera Silva-Carvalho; Bernadete Teixeira Ferreira-Carvalho; Paul Joseph Planet; Agnes Marie Sá Figueiredo

doi:10.1093/jac/dkac322

journal article Open Access Sep 29, 2022

High frequency of increased triclosan MIC among CC5 MRSA and risk of misclassification of the SCCmec into types

Alice Slotfeldt Viana Ana Maria Nunes Botelho Andries Feder Ahmed Magdi Moustafa Deborah Nascimento Santos Silva

Caroline Lopes Martini Adriana Lúcia Pires Ferreira Maria Cícera Silva-Carvalho Bernadete Teixeira Ferreira-Carvalho Paul Joseph Planet Agnes Marie Sá Figueiredo

Journal of Antimicrobial Chemotherapy Vol. 77 No. 12 pp. 3340-3348 · Oxford University Press (OUP)

View at Publisher Save 10.1093/jac/dkac322

Abstract

Abstract

Background
Typing of staphylococcal cassette chromosome mec (SCCmec) elements is commonly used for studies on the molecular epidemiology of MRSA.

Objectives
To perform an investigation centred on uncovering the reasons for misclassification of MRSA clonal complex 5 (CC5) SCCmec type II clinical isolates in our laboratory.

Methods
MRSA isolates from CC5 were subjected to WGS and SCCmec typing.

Results
This investigation led to the discovery that the classification failure was due to an insertion of IS1272 carrying the fabI gene on a transposable element (TnSha1) that confers increased MIC to the biocide triclosan. Genomic analysis revealed that fabI was present in 25% of the CC5 MRSA isolates sampled. The frequency of TnSha1 in our collection was much higher than that observed among publicly available genomes (0.8%; n = 24/3142 CC5 genomes). Phylogenetic analyses revealed that genomes in different CC5 clades carry TnSha1 inserted in different integration sites, suggesting that this transposon has entered CC5 MRSA genomes on multiple occasions. In at least two genotypes, ST5-SCCmecII-t539 and ST5-SCCmecII-t2666, TnSha1 seems to have entered prior to their divergence.

Conclusions
Our work highlights an important misclassification problem of SCCmecII in isolates harbouring TnSha1 when Boye’s method is used for typing, which could have important implications for molecular epidemiology of MRSA. The importance of increased-MIC phenotype is still a matter of controversy that deserves more study given the widespread use of triclosan in many countries. Our results suggest expanding prevalence that may indicate strong selection for this phenotype.

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Details

Published: Sep 29, 2022
Vol/Issue: 77(12)
Pages: 3340-3348
License: View

Authors

A

Alice Slotfeldt Viana

Department of Medical Microbiology, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ, 21941-902 , Brazil

A

Ana Maria Nunes Botelho

Department of Medical Microbiology, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ, 21941-902 , Brazil

A

Andries Feder

Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19104 , USA

A

Ahmed Magdi Moustafa

Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19104 , USA; Children’s Hospital of Philadelphia , Philadelphia, PA 19106 , USA

D

Deborah Nascimento Santos Silva

Department of Medical Microbiology, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ, 21941-902 , Brazil

C

Caroline Lopes Martini

Department of Medical Microbiology, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ, 21941-902 , Brazil

A

Adriana Lúcia Pires Ferreira

Department of Medical Microbiology, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ, 21941-902 , Brazil; Diagnósticos da América S.A. , Duque de Caxias, RJ, 25085-007 , Brazil

M

Maria Cícera Silva-Carvalho

Department of Medical Microbiology, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ, 21941-902 , Brazil

B

Bernadete Teixeira Ferreira-Carvalho

Department of Medical Microbiology, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ, 21941-902 , Brazil

P

Paul Joseph Planet

Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19104 , USA; Children’s Hospital of Philadelphia , Philadelphia, PA 19106 , USA

A

Agnes Marie Sá Figueiredo

Department of Medical Microbiology, Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ, 21941-902 , Brazil

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico

Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro

FAPERJ

CNPq

CAPES

Cite This Article

Alice Slotfeldt Viana, Ana Maria Nunes Botelho, Andries Feder, et al. (2022). High frequency of increased triclosan MIC among CC5 MRSA and risk of misclassification of the SCCmec into types. Journal of Antimicrobial Chemotherapy, 77(12), 3340-3348. https://doi.org/10.1093/jac/dkac322

High frequency of increased triclosan MIC among CC5 MRSA and risk of misclassification of the SCCmec into types

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