Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis

Huiying Cui; Bochao Zhang; Min Song; Shaolin Chen; Chaowen Xiao; Yunjia Tang; Johannes Liesche

doi:10.1093/jxb/eraa533

journal article Nov 12, 2020

Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis

Huiying Cui Bochao Zhang Min Song

Shaolin Chen

Chaowen Xiao

Yunjia Tang Johannes Liesche

Journal of Experimental Botany Vol. 72 No. 4 pp. 1073-1084 · Oxford University Press (OUP)

View at Publisher Save 10.1093/jxb/eraa533

Abstract

Abstract
The primary cell walls of plants provide mechanical strength while maintaining the flexibility needed for cell extension growth. Cell extension involves loosening the bonds between cellulose microfibrils, hemicelluloses and pectins. Pectins have been implicated in this process, but it remains unclear if this depends on the abundance of certain pectins, their modifications, and/or structure. Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy. Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content. While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo. The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity. It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.

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Details

Published: Nov 12, 2020
Vol/Issue: 72(4)
Pages: 1073-1084
License: View

Authors

H

Huiying Cui

College of Life Sciences, Northwest A&F University, Yangling, China; Biomass Energy Center for Arid and Semi-arid Lands, Northwest A&F University, Yangling, China

B

Bochao Zhang

College of Life Sciences, Northwest A&F University, Yangling, China

M

Min Song

College of Life Sciences, Northwest A&F University, Yangling, China; Biomass Energy Center for Arid and Semi-arid Lands, Northwest A&F University, Yangling, China

S

Shaolin Chen

College of Life Sciences, Northwest A&F University, Yangling, China; Biomass Energy Center for Arid and Semi-arid Lands, Northwest A&F University, Yangling, China

C

Chaowen Xiao

Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China

Y

Yunjia Tang

College of Life Sciences, Northwest A&F University, Yangling, China; State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, China

J

Johannes Liesche

College of Life Sciences, Northwest A&F University, Yangling, China; Biomass Energy Center for Arid and Semi-arid Lands, Northwest A&F University, Yangling, China

Funding

National Natural Science Foundation of China Award: 31700313

Cite This Article

Huiying Cui, Bochao Zhang, Min Song, et al. (2020). Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis. Journal of Experimental Botany, 72(4), 1073-1084. https://doi.org/10.1093/jxb/eraa533

Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis

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