Analysis of intraspecies diversity reveals a subset of highly variable plant immune receptors and predicts their binding sites

Daniil M Prigozhin; Ksenia V Krasileva

doi:10.1093/plcell/koab013

journal article Open Access Jan 25, 2021

Analysis of intraspecies diversity reveals a subset of highly variable plant immune receptors and predicts their binding sites

Daniil M Prigozhin

Ksenia V Krasileva

The Plant Cell Vol. 33 No. 4 pp. 998-1015 · Oxford University Press (OUP)

View at Publisher Save 10.1093/plcell/koab013

Abstract

Abstract
The evolution of recognition specificities by the immune system depends on the generation of receptor diversity and on connecting the binding of new antigens with the initiation of downstream signaling. In plant immunity, the innate Nucleotide-Binding Leucine-Rich Repeat (NLR) receptor family enables antigen binding and immune signaling. In this study, we surveyed the NLR complements of 62 ecotypes of Arabidopsis thaliana and 54 lines of Brachypodium distachyon and identified a limited number of NLR subfamilies that show high allelic diversity. We show that the predicted specificity-determining residues cluster on the surfaces of Leucine-Rich Repeat domains, but the locations of the clusters vary among NLR subfamilies. By comparing NLR phylogeny, allelic diversity, and known functions of the Arabidopsis NLRs, we formulate a hypothesis for the emergence of direct and indirect pathogen-sensing receptors and of the autoimmune NLRs. These findings reveal the recurring patterns of evolution of innate immunity and can inform NLR engineering efforts.

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Metrics

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Citations

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References

Details

Published: Jan 25, 2021
Vol/Issue: 33(4)
Pages: 998-1015
License: View

Authors

D

Daniil M Prigozhin

Berkeley Center for Structural Biology, Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA

K

Ksenia V Krasileva

Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA

Funding

National Institutes of Health

Howard Hughes Medical Institute

Gordon and Betty Moore Foundation Award: 8802

Two Blades Foundation together with Foundation for Food and Agriculture Research Award: CA19-SS-0000000046

The Berkeley Center for Structural Biology

Cite This Article

Daniil M Prigozhin, Ksenia V Krasileva (2021). Analysis of intraspecies diversity reveals a subset of highly variable plant immune receptors and predicts their binding sites. The Plant Cell, 33(4), 998-1015. https://doi.org/10.1093/plcell/koab013

Analysis of intraspecies diversity reveals a subset of highly variable plant immune receptors and predicts their binding sites

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