Transcription factors BBX11 and HY5 interdependently regulate the molecular and metabolic responses to UV-B

Nikhil Job; Maneesh Lingwan; Shyam Kumar Masakapalli; Sourav Datta

doi:10.1093/plphys/kiac195

journal article May 02, 2022

Transcription factors BBX11 and HY5 interdependently regulate the molecular and metabolic responses to UV-B

Nikhil Job

Maneesh Lingwan

Shyam Kumar Masakapalli

Sourav Datta

Plant Physiology Vol. 189 No. 4 pp. 2467-2480 · Oxford University Press (OUP)

View at Publisher Save 10.1093/plphys/kiac195

Abstract

Abstract
UV-B radiation acts as a developmental cue and a stress factor for plants, depending on dose. Activation of the transcription factor ELONGATED HYPOCOTYL 5 (HY5) in a UV RESISTANCE LOCUS 8 (UVR8)-dependent manner leads to the induction of a broad set of genes under UV-B. However, the underlying molecular mechanisms regulating this process are less understood. Here, we use molecular, biochemical, genetic, and metabolomic tools to identify the B-BOX transcription factor B-BOX PROTEIN 11 (BBX11) as a component of the molecular response to UV-B in Arabidopsis (Arabidopsis thaliana). BBX11 expression is induced by UV-B in a dose-dependent manner. Under low UV-B, BBX11 regulates hypocotyl growth suppression, whereas it protects plants exposed to high UV-B radiation by promoting the accumulation of photo-protective phenolics and antioxidants, and inducing DNA repair genes. Our genetic studies indicate that BBX11 regulates hypocotyl elongation under UV-B partially dependent on HY5. Overexpression of BBX11 can partially rescue the high UV-B sensitivity of hy5, suggesting that HY5-mediated UV-B stress tolerance is partially dependent on BBX11. HY5 regulates the UV-B-mediated induction of BBX11 by directly binding to its promoter. BBX11 reciprocally regulates the mRNA and protein levels of HY5. We report here the role of a BBX11-HY5 feedback loop in regulating photomorphogenesis and stress tolerance under UV-B.

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References

Details

Published: May 02, 2022
Vol/Issue: 189(4)
Pages: 2467-2480
License: View

Authors

N

Nikhil Job

Department of Biological Sciences, Indian Institute of Science Education and Research-Bhopal , Bhopal 462066, Madhya Pradesh, India

M

Maneesh Lingwan

BioX School of Basic Sciences, Indian Institute of Technology-Mandi , Mandi 175005, Himachal Pradesh, India

S

Shyam Kumar Masakapalli

BioX School of Basic Sciences, Indian Institute of Technology-Mandi , Mandi 175005, Himachal Pradesh, India

S

Sourav Datta

Department of Biological Sciences, Indian Institute of Science Education and Research-Bhopal , Bhopal 462066, Madhya Pradesh, India

Funding

DBT Award: BT/HRD/NBA-NWB/39/2020-21

CSIR

Cite This Article

Nikhil Job, Maneesh Lingwan, Shyam Kumar Masakapalli, et al. (2022). Transcription factors BBX11 and HY5 interdependently regulate the molecular and metabolic responses to UV-B. Plant Physiology, 189(4), 2467-2480. https://doi.org/10.1093/plphys/kiac195

Transcription factors BBX11 and HY5 interdependently regulate the molecular and metabolic responses to UV-B

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