Molecular insight into extreme copper resistance in the extremophilic archaeon ‘Ferroplasma acidarmanus’ Fer1

Craig Baker-Austin; Mark Dopson; Margaret Wexler; R. Gary Sawers; Philip L. Bond

doi:10.1099/mic.0.28076-0

journal article Aug 01, 2005

Molecular insight into extreme copper resistance in the extremophilic archaeon ‘Ferroplasma acidarmanus’ Fer1

Craig Baker-Austin Mark Dopson Margaret Wexler R. Gary Sawers

Philip L. Bond

Microbiology Vol. 151 No. 8 pp. 2637-2646 · Microbiology Society

View at Publisher Save 10.1099/mic.0.28076-0

Abstract

‘Ferroplasma acidarmanus’ strain Fer1 is an extremely acidophilic archaeon involved in the genesis of acid mine drainage, and was isolated from copper-contaminated mine solutions at Iron Mountain, CA, USA. Here, the initial proteomic and molecular investigation of Cu2+resistance in this archaeon is presented. Analysis of Cu2+toxicity via batch growth experiments and inhibition of oxygen uptake in the presence of ferrous iron demonstrated that Fer1 can grow and respire in the presence of 20 g Cu2+l−1. The Fer1 copper resistance (cop) loci [originally detected by Ettema, T. J. G., Huynen, M. A., de Vos, W. M. & van der Oost, J.Trends Biochem Sci28, 170–173 (2003)] include genes encoding a putative transcriptional regulator (copY), a putative metal-binding chaperone (copZ) and a putative copper-transporting P-type ATPase (copB). Transcription analyses demonstrated thatcopZandcopBare co-transcribed, and transcript levels were increased significantly in response to exposure to high levels of Cu2+, suggesting that the transport system is operating for copper efflux. Proteomic analysis of Fer1 cells exposed to Cu2+revealed the induction of stress proteins associated with protein folding and DNA repair (including RadA, thermosome and DnaK homologues), suggesting that ‘Ferroplasma acidarmanus’ Fer1 uses multiple mechanisms for resistance to high levels of copper.

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Details

Published: Aug 01, 2005
Vol/Issue: 151(8)
Pages: 2637-2646

Authors

C

Craig Baker-Austin

School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK

M

Mark Dopson

School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK

M

Margaret Wexler

School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK

R

R. Gary Sawers

Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK

P

Philip L. Bond

Centre for Ecology, Evolution and Conservation, University of East Anglia, Norwich NR4 7TJ, UK; School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK

Cite This Article

Craig Baker-Austin, Mark Dopson, Margaret Wexler, et al. (2005). Molecular insight into extreme copper resistance in the extremophilic archaeon ‘Ferroplasma acidarmanus’ Fer1. Microbiology, 151(8), 2637-2646. https://doi.org/10.1099/mic.0.28076-0

Molecular insight into extreme copper resistance in the extremophilic archaeon ‘Ferroplasma acidarmanus’ Fer1

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