Succinate-mediated catabolite repression control on the production of glycine betaine catabolic enzymes in Pseudomonas aeruginosa PAO1 under low and elevated salinities

Farès Diab; Théophile Bernard; Alexis Bazire; Dominique Haras; Carlos Blanco; Mohamed Jebbar

doi:10.1099/mic.0.28652-0

journal article May 01, 2006

Succinate-mediated catabolite repression control on the production of glycine betaine catabolic enzymes in Pseudomonas aeruginosa PAO1 under low and elevated salinities

Farès Diab Théophile Bernard Alexis Bazire

Dominique Haras Carlos Blanco

Mohamed Jebbar

Microbiology Vol. 152 No. 5 pp. 1395-1406 · Microbiology Society

View at Publisher Save 10.1099/mic.0.28652-0

Abstract

Glycine betaine (GB) and its immediate precursors choline and carnitine, dimethylsulfonioacetate, dimethylsulfoniopropionate, ectoine and proline were effective osmoprotectants for Pseudomonas aeruginosa, but pipecolate, trehalose and sucrose had no osmoprotective effect. GB was accumulated stably or transiently when succinate or glucose, respectively, was used as a carbon and energy source. The catabolite repression mediated by succinate occurred at both low and high salinities, and it did not involve the global regulators Vfr and Crc. A proteomic analysis showed that at least 21 proteins were induced when GB was used as a carbon and energy source, and provided evidence that succinate repressed the synthesis of all these proteins. Many of the proteins induced by GB (sarcosine oxidase, serine hydroxymethyltransferase and serine dehydratase) are involved in GB catabolism. In addition, GB uptake was stimulated at high medium osmolalities but it was insensitive to catabolite repression by succinate. Despite its ability to inhibit betaine catabolism, succinate did not allow any better growth of P. aeruginosa cells under hyperosmotic constraint. Conversely, as observed for cells supplied with glucose, a transient accumulation of GB was sufficient to provide a significant cell osmoprotection.

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Details

Published: May 01, 2006
Vol/Issue: 152(5)
Pages: 1395-1406

Authors

F

Farès Diab

Departement Osmorégulation chez les Bactéries, UMR-CNRS 6026, Université de Rennes 1, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France

T

Théophile Bernard

Departement Osmorégulation chez les Bactéries, UMR-CNRS 6026, Université de Rennes 1, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France

A

Alexis Bazire

Laboratoire de Biotechnologie et Chimie Marines, EA 3884, Université de Bretagne Sud, Lorient, France

D

Dominique Haras

Laboratoire de Biotechnologie et Chimie Marines, EA 3884, Université de Bretagne Sud, Lorient, France

C

Carlos Blanco

Departement Osmorégulation chez les Bactéries, UMR-CNRS 6026, Université de Rennes 1, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France

M

Mohamed Jebbar

Departement Osmorégulation chez les Bactéries, UMR-CNRS 6026, Université de Rennes 1, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France

Cite This Article

Farès Diab, Théophile Bernard, Alexis Bazire, et al. (2006). Succinate-mediated catabolite repression control on the production of glycine betaine catabolic enzymes in Pseudomonas aeruginosa PAO1 under low and elevated salinities. Microbiology, 152(5), 1395-1406. https://doi.org/10.1099/mic.0.28652-0

Succinate-mediated catabolite repression control on the production of glycine betaine catabolic enzymes in Pseudomonas aeruginosa PAO1 under low and elevated salinities

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