WRKY75 Transcription Factor Is a Modulator of Phosphate Acquisition and Root Development in Arabidopsis

Ballachanda N. Devaiah; Athikkattuvalasu S. Karthikeyan; Kashchandra G. Raghothama

doi:10.1104/pp.106.093971

journal article Open Access Feb 23, 2007

WRKY75 Transcription Factor Is a Modulator of Phosphate Acquisition and Root Development in Arabidopsis

Ballachanda N. Devaiah Athikkattuvalasu S. Karthikeyan Kashchandra G. Raghothama

Plant Physiology Vol. 143 No. 4 pp. 1789-1801 · Oxford University Press (OUP)

View at Publisher Save 10.1104/pp.106.093971

Abstract

AbstractPhosphate (Pi) deficiency limits plant growth and development, resulting in adaptive stress responses. Among the molecular determinants of Pi stress responses, transcription factors play a critical role in regulating adaptive mechanisms. WRKY75 is one of several transcription factors induced during Pi deprivation. In this study, we evaluated the role of the WRKY75 transcription factor in regulating Pi starvation responses in Arabidopsis (Arabidopsis thaliana). WRKY75 was found to be nuclear localized and induced differentially in the plant during Pi deficiency. Suppression of WRKY75 expression through RNAi silencing resulted in early accumulation of anthocyanin, indicating that the RNAi plants were more susceptible to Pi stress. Further analysis revealed that the expression of several genes involved in Pi starvation responses, including phosphatases, Mt4/TPS1-like genes, and high-affinity Pi transporters, was decreased when WRKY75 was suppressed. Consequently, Pi uptake of the mutant plant was also decreased during Pi starvation. In addition, when WRKY75 expression was suppressed, lateral root length and number, as well as root hair number, were significantly increased. However, changes in the root architecture were obvious under both Pi-sufficient and Pi-deficient conditions. This indicates that the regulatory effect of WRKY75 on root architecture could be independent of the Pi status of the plant. Together, these results suggest that WRKY75 is a modulator of Pi starvation responses as well as root development. WRKY75 is the first member of the WRKY transcription factor family reported to be involved in regulating a nutrient starvation response and root development.

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Details

Published: Feb 23, 2007
Vol/Issue: 143(4)
Pages: 1789-1801
License: View

Authors

B

Ballachanda N. Devaiah

Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907–1165

A

Athikkattuvalasu S. Karthikeyan

Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907–1165

K

Kashchandra G. Raghothama

Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907–1165

Cite This Article

Ballachanda N. Devaiah, Athikkattuvalasu S. Karthikeyan, Kashchandra G. Raghothama (2007). WRKY75 Transcription Factor Is a Modulator of Phosphate Acquisition and Root Development in Arabidopsis. Plant Physiology, 143(4), 1789-1801. https://doi.org/10.1104/pp.106.093971

WRKY75 Transcription Factor Is a Modulator of Phosphate Acquisition and Root Development in Arabidopsis

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