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journal article Jun 11, 2013

Cytology of infection, development and expression of resistance to Plasmodiophora brassicae in canola

A. Deora B. D. Gossen M. R. McDonald
Annals of Applied Biology Vol. 163 No. 1 pp. 56-71 · Wiley
View at Publisher Save 10.1111/aab.12033
Abstract
AbstractThe timing and expression of resistance to four isolates of Plasmodiophora brassicae, collected from research sites where pathotypes 2, 3, 5 and 6 (Williams' system) had been dominant when characterised in 2006, were assessed in four new commercial cultivars of canola (Brassica napus) with resistance to clubroot. Each of the resistant cultivars was highly resistant to all four of the isolates, and there was no difference in their response to infection. Root hair infection occurred at high levels, but pathogen development occurred more slowly than in a susceptible cultivar (control). Secondary infection and development in cortical cells was severely inhibited in each of the resistant cultivars; only a few bi‐nucleated plasmodia were observed at 12 days after inoculation (DAI), and plasmodia were rarely observed at 18 and 24 DAI. In contrast, development in the susceptible cultivar had progressed to resting spores by 24 DAI. A dense ring of accumulated reactive oxygen species (ROS) was observed in the endodermis, pericycle and vascular cambium of non‐inoculated controls and inoculated plants of the resistant cultivars. However, the ROS ring disappeared rapidly in infected plants of the susceptible cultivar. Plasmodia invaded the stele of susceptible roots by preferentially colonising the xylem parenchyma cells. Expansion and enlargement of lignified xylem cells was observed by 35 DAI. The absence of any specific points of ROS accumulation or lignification of epidermal or cortical cells in the resistant cultivars indicates that a hypersensitive response is not the main mechanism of resistance in these lines. The uniform response of these resistant cultivars to the four isolates of P. brassicae indicates that the resistance in each cultivar may be conditioned by a gene(s) from a single source that confers broad resistance, because most sources of resistance to P. brassicae are pathotype specific.
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References
47
[6]
Crute I.R. (1986)
[10]
Diederichsen E. (2003)
[12]
The Occurrence and Economic Impact of Plasmodiophora brassicae and Clubroot Disease

Geoffrey R. Dixon

Journal of Plant Growth Regulation 10.1007/s00344-009-9090-y
[22]
Keen N.T. "Synthesis and degradation of starch and lipids following infection of cabbage by Plasmodiophora brassicae" Phytopathology (1969)
[30]
MorgnerM.(1995) Mikroskopische Untersuchungen der Reaktionen empfindlicher und resistenter Genotypen verschiedener Brassica‐Arten gegenüberPlasmodiophora brassicae(Wor.). PhD Dissertation. Institut für Angewandte Genetik Freie Universität Berlin Berlin.
[31]
Morgner M. "Quantitative determination of colonization of Plasmodiophora brassicae (Wor.) in Brassica napus and Brassica oleracea" Cruciferae Newsletter (1995)
[33]
PetersonR.L. PetersonC.A. MelvilleL.H.(2008) Cell types in simple tissues.Teaching plant anatomy through creative laboratory exercises. pp. 31–39. St Paul MN USA: APS Press.
[35]
Punja Z.K. (2004)
[45]
Wallenhamar A.C. (1999)
[46]
Williams P.H. "A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga" Phytopathology (1966)
[47]
Yeung E.C. (1998)
Metrics
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Citations
47
References
Details
Published
Jun 11, 2013
Vol/Issue
163(1)
Pages
56-71
License
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Authors
A
A. Deora

Agriculture and Agri‐Food Canada Saskatoon SK S7N 0X2 Canada; Department of Plant Agriculture University of Guelph Guelph ON N1G 2 W1 Canada

B
B. D. Gossen

Agriculture and Agri‐Food Canada Saskatoon SK S7N 0X2 Canada

M
M. R. McDonald

Department of Plant Agriculture University of Guelph Guelph ON N1G 2 W1 Canada

Cite This Article
A. Deora, B. D. Gossen, M. R. McDonald (2013). Cytology of infection, development and expression of resistance to Plasmodiophora brassicae in canola. Annals of Applied Biology, 163(1), 56-71. https://doi.org/10.1111/aab.12033
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