Impact of sperm characteristics on time‐lapse embryo morphokinetic parameters and clinical outcome of conventional in vitro fertilization

Stefka Nikolova; Dimitar Parvanov; Vilyana Georgieva; Ivka Ivanova; Rumiana Ganeva; Georgi Stamenov

doi:10.1111/andr.12781

journal article Mar 30, 2020

Impact of sperm characteristics on time‐lapse embryo morphokinetic parameters and clinical outcome of conventional in vitro fertilization

Stefka Nikolova Dimitar Parvanov

Vilyana Georgieva Ivka Ivanova Rumiana Ganeva Georgi Stamenov

Andrology Vol. 8 No. 5 pp. 1107-1116 · Wiley

View at Publisher Save 10.1111/andr.12781

Abstract

AbstractBackgroundSperm abnormalities may negatively affect embryo development.ObjectivesTo determine the influence of sperm abnormalities (morphology, motility, DNA fragmentation) on embryo morphokinetic variables and clinical outcome of conventional IVF.Materials and methodsParticipants were 86 couples undergoing in vitro fertilization (IVF). Sperm morphology was evaluated according to the strict criteria proposed by Kruger/Tygerberg. CASA system was applied for sperm motility assessment. Sperm DNA fragmentation was assessed by the chromatin structure assay (SCSA). Morphokinetic parameters were determined in 223 embryos obtained from conventional IVF only and cultured in a single‐step medium using time‐lapse imaging technology.ResultsTime‐lapse variables from the initial embryo development, such as time of pronuclei fading (tPNf) and time for two cells (t2), were those more strongly related with abnormalities of sperm motility, morphology, and DNA fragmentation. Sperm morphological abnormalities rather than sperm motility were more closely associated with embryo morphokinetics. Sperm head defects were mainly correlated with the last stages of embryonic development (t9 to tHB), sperm midpiece defects with intermediate cleaving embryos (t5‐t9), and sperm tail defects with the initial stages of embryonic development (tPNa‐t4). Excess residual cytoplasm was positively correlated with all embryo morphokinetic parameters except t2 and tM. Absence of acrosomes, pinheads, coiled tails, and multiple sperm morphological defects correlated negatively with time‐lapse embryo morphokinetic variables.DiscussionA large number of sperm‐related variables, including frequency of specific morphological defects, morphological indexes, DNA fragmentation and motility, and time‐lapse embryo variables, such as time intervals based mainly of 15 time points were recorded.ConclusionThere were strong associations between specific sperm defects of the head, midpiece, and tail with certain stages of embryonic development from observation of pronuclei to the hatched blastocyst. Coiled tail, cumulative head defects, and multiple abnormalities index (MAI) were associated both with embryo morphokinetics and the implantation success.

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Details

Published: Mar 30, 2020
Vol/Issue: 8(5)
Pages: 1107-1116
License: View

Authors

S

Stefka Nikolova

Embryology Department Nadezhda Women’s Health Hospital Sofia Bulgaria

D

Dimitar Parvanov

Research Department Nadezhda Women’s Health Hospital Sofia Bulgaria

V

Vilyana Georgieva

Andrology Department Nadezhda Women’s Health Hospital Sofia Bulgaria

I

Ivka Ivanova

Embryology Department Nadezhda Women’s Health Hospital Sofia Bulgaria

R

Rumiana Ganeva

Research Department Nadezhda Women’s Health Hospital Sofia Bulgaria

G

Georgi Stamenov

Obsterics and Gynecology Department Nadezhda Women’s Health Hospital Sofia Bulgaria

Cite This Article

Stefka Nikolova, Dimitar Parvanov, Vilyana Georgieva, et al. (2020). Impact of sperm characteristics on time‐lapse embryo morphokinetic parameters and clinical outcome of conventional in vitro fertilization. Andrology, 8(5), 1107-1116. https://doi.org/10.1111/andr.12781

Impact of sperm characteristics on time‐lapse embryo morphokinetic parameters and clinical outcome of conventional in vitro fertilization

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