Genomic epidemiology and antimicrobial resistance determinants of Neisseria gonorrhoeae isolates from Ukraine, 2013–2018

Iryna Boiko; Daniel Golparian; Susanne Jacobsson; Inna Krynytska; Arkadii Frankenberg; Tetiana Shevchenko; Magnus Unemo

doi:10.1111/apm.13060

journal article Open Access Jun 28, 2020

Genomic epidemiology and antimicrobial resistance determinants of Neisseria gonorrhoeae isolates from Ukraine, 2013–2018

Iryna Boiko Daniel Golparian Susanne Jacobsson Inna Krynytska Arkadii Frankenberg Tetiana Shevchenko

Magnus Unemo

APMIS Vol. 128 No. 7 pp. 465-475 · Wiley

View at Publisher Save 10.1111/apm.13060

Abstract

Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is a major health threat compromising the gonorrhoea treatment globally. AMR surveillance including whole genome sequencing (WGS)‐based epidemiology provides ideal resolution to identify and describe AMR gonococcal clones, AMR determinants and populations, which can inform management guidelines and antimicrobial stewardship policies. Our aims were to, for the first time, elucidate the WGS‐based epidemiology and characterize AMR determinants of gonococcal strains spreading in Ukraine, 2013–2018. Gonococcal isolates (n = 150) from Ternopil and Dnipro, Ukraine (2013–2018), were subjected to AMR testing (Etest) for eight antimicrobials and WGS. Overall, 11.3% of isolates were resistant to ciprofloxacin, 6.0% to tetracycline, and 0.7% to benzylpenicillin. No isolates were resistant to azithromycin, spectinomycin, ceftriaxone, or cefixime, but one isolate was bordering resistance to both cephalosporins. Twenty‐five MLST STs, 50 NG‐MAST STs, and 34 NG‐STAR types were identified. The phylogenomic analysis revealed six main clusters, mostly associated with the internationally described multidrug‐susceptible gonococcal lineage. Resistance to ciprofloxacin was associated with GyrA S91F and ParC S87R mutations; tetracyclines with rpsJ V57M and tetM; penicillins with mosaic penA‐34.001 and β‐lactamase; mtrR; PorB1b G101D, and PBP1 L421P mutations. One isolate of the multidrug‐resistant NG‐MAST ST1407, MLST ST1901 was found, which was bordering resistance to ceftriaxone and cefixime. The antimicrobial susceptibility of gonococcal strains spreading in Ternopil and Dnipro, Ukraine, in 2013–2018 was surprisingly high. Continued and expanded gonococcal AMR surveillance, ideally including WGS, in Ukraine is essential. This could inform action plans and public health policies to control the spread of AMR gonococcal strains in Ukraine.

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Details

Published: Jun 28, 2020
Vol/Issue: 128(7)
Pages: 465-475
License: View

Authors

I

Iryna Boiko

Department of Functional and Laboratory Diagnostics I. Horbachevsky Ternopil National Medical University Ternopil Ukraine; WHO Collaborating Centre for Gonorrhoea and other STIs National Reference Laboratory for STIs Department of Laboratory Medicine Faculty of Medicine and Health Örebro University Örebro Sweden

D

Daniel Golparian

WHO Collaborating Centre for Gonorrhoea and other STIs National Reference Laboratory for STIs Department of Laboratory Medicine Faculty of Medicine and Health Örebro University Örebro Sweden

S

Susanne Jacobsson

WHO Collaborating Centre for Gonorrhoea and other STIs National Reference Laboratory for STIs Department of Laboratory Medicine Faculty of Medicine and Health Örebro University Örebro Sweden

I

Inna Krynytska

Department of Functional and Laboratory Diagnostics I. Horbachevsky Ternopil National Medical University Ternopil Ukraine

A

Arkadii Frankenberg

Dnipro Regional Dermatovenerologic Dispensary Dnipro Ukraine

T

Tetiana Shevchenko

Department of General Medicine with a Course of Physical Therapy Faculty of Medical Technologies of Diagnostics and Rehabilitation Oles Honchar Dnipro National University Dnipro Ukraine

M

Magnus Unemo

WHO Collaborating Centre for Gonorrhoea and other STIs National Reference Laboratory for STIs Department of Laboratory Medicine Faculty of Medicine and Health Örebro University Örebro Sweden

Cite This Article

Iryna Boiko, Daniel Golparian, Susanne Jacobsson, et al. (2020). Genomic epidemiology and antimicrobial resistance determinants of Neisseria gonorrhoeae isolates from Ukraine, 2013–2018. APMIS, 128(7), 465-475. https://doi.org/10.1111/apm.13060

Genomic epidemiology and antimicrobial resistance determinants of Neisseria gonorrhoeae isolates from Ukraine, 2013–2018

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