The features of mucosa‐associated microbiota in primary sclerosing cholangitis
Background
Little is known about the role of the microbiome in primary sclerosing cholangitis.
Aim
To explore the mucosa‐associated microbiota in primary sclerosing cholangitis (PSC) patients across different locations in the gut, and to compare it with inflammatory bowel disease (IBD)‐only patients and healthy controls.
Methods
Biopsies from the terminal ileum, right colon, and left colon were collected from patients and healthy controls undergoing colonoscopy. Microbiota profiling using bacterial 16S rRNA sequencing was performed on all biopsies.
Results
Forty‐four patients were recruited: 20 with PSC (19 with PSC‐IBD and one with PSC‐only), 15 with IBD‐only and nine healthy controls. The overall microbiome profile was similar throughout different locations in the gut. No differences in the global microbiome profile were found. However, we observed significant PSC‐associated enrichment in
Barnesiellaceae
at the family level, and in
Blautia
and an unidentified
Barnesiellaceae
at the genus level. At the operational taxa unit level, most shifts in PSC were observed in
Clostridiales
and
Bacteroidales
orders, with approximately 86% of shifts occurring within the former order.
Conclusions
The overall microbiota profile was similar across multiple locations in the gut from the same individual regardless of disease status. In this study, the mucosa associated‐microbiota of patients with primary sclerosing cholangitis was characterised by enrichment of
Blautia
and
Barnesiellaceae
and by major shifts in operational taxa units within
Clostridiales
order.
No keywords indexed for this article. Browse by subject →
Noortje G. Rossen, Susana Fuentes, Kirsten Boonstra et al.
Jason M. Ridlon, Dae J. Kang, Phillip B. Hylemon et al.
Jason M. Ridlon, Dae-Joong Kang, Phillip B. Hylemon
Kjersti Aagaard, Kevin Riehle, Jun Ma et al.
J Gregory Caporaso, Justin Kuczynski, Jesse Stombaugh et al.
Xochitl C Morgan, Timothy L Tickle, Harry Sokol et al.
Seiko Narushima, Kikuji Itoh, Yukiko Miyamoto et al.
Koji Atarashi, Takeshi Tanoue, Kenshiro Oshima et al.
K.B.M. Saiful Islam, Satoru Fukiya, Masahito Hagio et al.
Zhen-Jiao Li, Hong-Zhong Gou · 2022
Mirco Vacca, Giuseppe Celano · 2020
Rebecca Little, Eytan Wine · 2020
J Torres, C Palmela · 2017
Xiaojun Jiang, Tom H. Karlsen · 2017
- Published
- Feb 09, 2016
- Vol/Issue
- 43(7)
- Pages
- 790-801
- License
- View
You May Also Like
G. Vernon, A. Baranova · 2011
2,464 citations
H. M. HAMER, D. JONKERS · 2007
2,316 citations
C. Y. FRANCIS, J. Morris · 1997
1,407 citations
Yuan Tian, Long Rong · 2020
652 citations
S. Ben-Horin, Y. Chowers · 2011
545 citations