Independent effect of polymeric nanoparticle zeta potential/surface charge, on their cytotoxicity and affinity to cells

Xiao‐Ru Shao; Xue‐Qin Wei; Xu Song; Li‐Ying Hao; Xiao‐Xiao Cai; Zhi‐Rong Zhang; Qiang Peng; Yun‐Feng Lin

doi:10.1111/cpr.12192

journal article May 27, 2015

Independent effect of polymeric nanoparticle zeta potential/surface charge, on their cytotoxicity and affinity to cells

Xiao‐Ru Shao Xue‐Qin Wei Xu Song

Li‐Ying Hao Xiao‐Xiao Cai Zhi‐Rong Zhang Qiang Peng

Yun‐Feng Lin

Cell Proliferation Vol. 48 No. 4 pp. 465-474 · Wiley

View at Publisher Save 10.1111/cpr.12192

Abstract

AbstractObjectivesUp to now, little research has been focussed on discovering how zeta potential independently affects polymeric nanoparticle (NP) cytotoxicity.MethodsPolymeric nanoparticles of gradient zeta potential ranging from −30 mv to +40 mv were fabricated using the same poly‐3‐hydroxybutyrate‐co‐3‐hydroxyhexanoate (PHBHHx) biopolymer. Interaction forces between nanoparticles and cells were measured by atomic force microscopy (AFM). Cytotoxicity of the nanoparticles to cells was investigated by using MTT (3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide) assay.ResultsFour kinds of nanoparticle with similar sizes and gradient zeta potentials, were fabricated. Those with positive surface charges were found to be more toxic than those with negative surface charges. Positively charged nanoparticles or nanoparticles with higher ‘like’ charges, offered higher interaction force with cells.ConclusionThis work proposes a novel approach for investigating interaction between NPs and cells, and discloses the importance of controlling zeta potential in developing NPs‐based formulations in the future.

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Details

Published: May 27, 2015
Vol/Issue: 48(4)
Pages: 465-474
License: View

Authors

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Xiao‐Ru Shao