Macrophages enhance regeneration of lateral line neuromast derived from interneuromast cells through TGF‐β in zebrafish

Wei‐Lin Hsu; Yu‐Chi Lin; Meng‐Ju Lin; Shyh‐Jye Lee

doi:10.1111/dgd.12911

journal article Jan 28, 2024

Macrophages enhance regeneration of lateral line neuromast derived from interneuromast cells through TGF‐β in zebrafish

Wei‐Lin Hsu Yu‐Chi Lin Meng‐Ju Lin Shyh‐Jye Lee

Development, Growth & Differentiation Vol. 66 No. 2 pp. 133-144 · Wiley

View at Publisher Save 10.1111/dgd.12911

Abstract

AbstractMacrophages play a pivotal role in the response to injury, contributing significantly to the repair and regrowth of damaged tissues. The external lateral line system in aquatic organisms offers a practical model for studying regeneration, featuring interneuromast cells connecting sensory neuromasts. Under normal conditions, these cells remain dormant, but their transformation into neuromasts occurs when overcoming inhibitory signals from Schwann cells and posterior lateral line nerves. The mechanism enabling interneuromast cells to evade inhibition by Schwann cells remains unclear. Previous observations suggest that macrophages physically interact with neuromasts, nerves, and Schwann cells during regeneration. This interaction leads to the regeneration of neuromasts in a subset of zebrafish with ablated neuromasts. To explore whether macrophages achieve this effect through secreted cytokines, we conducted experiments involving tail amputation in zebrafish larvae and tested the impact of cytokine inhibitors on neuromast regeneration. Most injured larvae remarkably regenerated a neuromast within 4 days post‐amputation. Intriguingly, removal of macrophages and inhibition of the anti‐inflammatory cytokine transforming growth factor‐beta (TGF‐β) significantly delayed neuromast regeneration. Conversely, inhibition of the pro‐inflammatory cytokines interleukin‐6 (IL‐6) and tumor necrosis factor‐alpha (TNF‐α) had minor effects on the regeneration process. This study provides insights into how macrophages activate interneuromast cells, elucidating the pathways underlying neuromast regeneration.

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Details

Published: Jan 28, 2024
Vol/Issue: 66(2)
Pages: 133-144
License: View

Authors

W

Wei‐Lin Hsu

Department of Life Science National Taiwan University Taipei Taiwan

Y

Yu‐Chi Lin

Department of Life Science National Taiwan University Taipei Taiwan

M

Meng‐Ju Lin

Department of Life Science National Taiwan University Taipei Taiwan

S

Shyh‐Jye Lee

Department of Life Science National Taiwan University Taipei Taiwan; Research Center for Developmental Biology and Regenerative Medicine National Taiwan University Taipei Taiwan; Center for Biotechnology, National Taiwan University Taipei Taiwan

Funding

Ministry of Science and Technology, Taiwan Award: MOST 108‐2311‐B‐002‐015

Cite This Article

Wei‐Lin Hsu, Yu‐Chi Lin, Meng‐Ju Lin, et al. (2024). Macrophages enhance regeneration of lateral line neuromast derived from interneuromast cells through TGF‐β in zebrafish. Development, Growth & Differentiation, 66(2), 133-144. https://doi.org/10.1111/dgd.12911

Macrophages enhance regeneration of lateral line neuromast derived from interneuromast cells through TGF‐β in zebrafish

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