Trichoderma reesei XYN VI – a novel appendage‐dependent eukaryotic glucuronoxylan hydrolase

Peter Biely; Vladimír Puchart; Mary Ann Stringer; Kristian B.R. Mørkeberg Krogh

doi:10.1111/febs.12925

journal article Jul 30, 2014

Trichoderma reesei XYN VI – a novel appendage‐dependent eukaryotic glucuronoxylan hydrolase

Peter Biely Vladimír Puchart

Mary Ann Stringer Kristian B.R. Mørkeberg Krogh

The FEBS Journal Vol. 281 No. 17 pp. 3894-3903 · Wiley

View at Publisher Save 10.1111/febs.12925

Abstract

Expression of a Trichoderma reesei gene coding for a putative GH30 xylanase in Aspergillus oryzae led to isolation and purification of a novel xylanase exhibiting catalytic properties different from those of the previously characterized GH30 xylanase XYN IV of T. reesei. The novel enzyme, named XYN VI, exhibited catalytic properties similar to appendage‐dependent GH30 glucuronoxylanases previously recognized only in bacteria. XYN VI showed high specific activity only on xylans or xylooligosaccharides containing 4‐O‐methyl‐d‐glucuronic acid or d‐glucuronic acid side substituents. The cleavage of the main chain takes place primarily at the second glycosidic linkage from the branch towards the reducing end of the polysaccharides or aldouronic acids. These catalytic properties resemble bacterial GH30 glucuronoxylanases, although the recognition of the uronic acid side chains by XYN VI is apparently based on interaction of the substrate with other amino acids. Moreover, in contrast to bacterial enzymes, XYN VI is also capable of slower but significant cleavage of unsubstituted parts of xylan and acidic xylooligosaccharides. The data point to a great catalytic diversity of xylanases produced by the most extensively studied cellulolytic fungus.

Topics

No keywords indexed for this article. Browse by subject →

References

34

[1]

Biely P "Resolution of glycanases of Trichoderma reesei with respect to cellulose and xylan degradation" Biotechnol Appl Biochem (1988)

[2]

10.1016/0141-0229(92)90128-b

[3]

10.1038/nbt1192-1461

[4]

10.1007/s002530051237

[5]

10.1007/s002530000410

[6]

10.1007/s00253-006-0365-y

[7]

10.1186/1475-2859-11-134

[8]

10.1111/j.1432-1033.1991.tb21062.x

[9]

SaloheimoM Siika‐AhoM TenkanenM&PenttiläM(2003)Xylanase from Trichoderma reesei method for production thereof and methods employing this enzyme. US Patent 6 555 335 B1.

[10]

10.1111/febs.12069

[11]

Consolidation of glycosyl hydrolase family 30: A dual domain 4/7 hydrolase family consisting of two structurally distinct groups

Franz J. St John, Javier M. González, Edwin Pozharski

FEBS Letters 10.1016/j.febslet.2010.09.051

[12]

10.1016/s0021-9258(18)38151-1

[13]

Characterization of XynC from Bacillus subtilis subsp. subtilis Strain 168 and Analysis of Its Role in Depolymerization of Glucuronoxylan

Franz J. St. John, John D. Rice, James F. Preston

Journal of Bacteriology 10.1128/jb.01283-06

[14]

10.1111/j.1742-4658.2007.05710.x

[15]

Characterization of a Family GH5 Xylanase with Activity on Neutral Oligosaccharides and Evaluation as a Pulp Bleaching Aid

Oscar Gallardo, María Fernández-Fernández, Cristina Valls et al.

Applied and Environmental Microbiology 10.1128/aem.00871-10

[16]

10.1007/s00253-009-2410-0

[17]

10.1186/1754-6834-6-127

[18]

The carbohydrate-active enzymes database (CAZy) in 2013

Vincent Lombard, Hemalatha Golaconda Ramulu, Elodie Drula et al.

Nucleic Acids Research 10.1093/nar/gkt1178

[19]

10.1016/s0168-1656(97)00096-5

[20]

10.1016/j.jbiotec.2006.12.011

[21]

10.1071/ap03057

[22]

10.1021/bi034144c

[23]

10.1111/j.1742-4658.2011.08127.x

[24]

BudolfsenG JensenMT Heldt‐HansenHP StringerMA&LangeL(2004)Method of preparing a heat‐treated product. International Patent Application No. WO 2004/032648.

[25]

McBrayerB ShaghasiT&VlasenkoE(2011)Compositions for saccharification of cellulosic material. International Patent Application No. WO 2011/057140.

[26]

10.1038/nbt1288-1419

[27]

StringerMA&McBrayerB(2012)Polypeptides having cellobiohydrolase activity and polynucleotides encoding same. International Patent Application No. WO 2012/103350.

[28]

10.1006/abio.2000.4810

[29]

10.1016/0076-6879(88)60165-0

[30]

10.3891/acta.chem.scand.14-0742

[31]

10.1016/j.jcs.2007.07.009

[32]

10.1021/ac60155a072

[33]

10.1016/s0304-4165(03)00130-2

[34]

10.1016/0003-2697(80)90523-0

Cited By

51

Crystal structure of reducing‐end xylose‐releasing exoxylanase in subfamily 7 of glycoside hydrolase family 30

Yusuke Nakamichi, Masahiro Watanabe · 2023

Proteins: Structure, Function, and...

The first crystal structure of a xylobiose‐bound xylobiohydrolase with high functional specificity from the bacterial glycoside hydrolase family 30, subfamily 10

Franz J. St John, Casey Crooks · 2022

FEBS Letters

Xylanases of glycoside hydrolase family 30 – An overview

Vladimír Puchart, Katarína Šuchová · 2021

Biotechnology Advances

Feruloylated Arabinoxylan and Oligosaccharides: Chemistry, Nutritional Functions, and Options for Enzymatic Modification

Shang Lin, Jane W. Agger · 2021

Annual Review of Food Science and T...

A novel bacterial GH30 xylobiohydrolase from Hungateiclostridium clariflavum

Katarína Šuchová, Vladimír Puchart · 2020

Applied Microbiology and Biotechnol...

A novel GH30 xylobiohydrolase from Acremonium alcalophilum releasing xylobiose from the non-reducing end

Katarína Šuchová, Vladimír Puchart · 2020

Enzyme and Microbial Technology

Crystal structure of GH30-7 endoxylanase C from the filamentous fungusTalaromyces cellulolyticus

Yusuke Nakamichi, Tatsuya Fujii · 2020

Acta Crystallographica Section F St...

Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei

Robert H. Bischof, Jonas Ramoni · 2016

Microbial Cell Factories

Metrics

51

Citations

34

References

Details

Published: Jul 30, 2014
Vol/Issue: 281(17)
Pages: 3894-3903
License: View

Authors

P

Peter Biely

Institute of Chemistry Center of Glycomics Slovak Academy of Sciences Bratislava Slovakia

V

Vladimír Puchart

Institute of Chemistry Center of Glycomics Slovak Academy of Sciences Bratislava Slovakia

M

Mary Ann Stringer

Novozymes A/S Bagsvaerd Denmark

K

Kristian B.R. Mørkeberg Krogh

Novozymes A/S Bagsvaerd Denmark

Funding

Slovak Research and Development Agency Award: APVV 0602 12

Cite This Article

Peter Biely, Vladimír Puchart, Mary Ann Stringer, et al. (2014). Trichoderma reesei XYN VI – a novel appendage‐dependent eukaryotic glucuronoxylan hydrolase. The FEBS Journal, 281(17), 3894-3903. https://doi.org/10.1111/febs.12925

Trichoderma reesei XYN VI – a novel appendage‐dependent eukaryotic glucuronoxylan hydrolase

You May Also Like