Ribosome‐bound Pub1 modulates stop codon decoding during translation termination in yeast

Valery N. Urakov; Olga V. Mitkevich; Irina V. Safenkova; Michael D. Ter‐Avanesyan

doi:10.1111/febs.14099

journal article May 29, 2017

Ribosome‐bound Pub1 modulates stop codon decoding during translation termination in yeast

Valery N. Urakov Olga V. Mitkevich Irina V. Safenkova Michael D. Ter‐Avanesyan

The FEBS Journal Vol. 284 No. 12 pp. 1914-1930 · Wiley

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Abstract

In eukaryotes, termination of translation is controlled by polypeptide chain release factors eRF1 and eRF3, of which the former recognizes nonsense codons, while the latter interacts with eRF1 and stimulates polypeptide release from the ribosome in a GTP‐ dependent manner, and ABCE1, which facilitates ribosome recycling. In this work, we demonstrate that Pub1, a yeast protein known to be involved in stress granule formation, regulation of gene expression, and organization of the tubulin cytoskeleton, also plays a role in translation termination. Pub1 was shown to bind to ribosomes independent of eRF1 and eRF3 and to interact with the N‐terminal glutamine‐/asparagine‐rich prion domain of eRF3 via its short C‐terminal glutamine‐rich tract. High velocity sedimentation in sucrose gradient demonstrated that Pub1 was preferentially associated with heavy polysomes enriched with terminating ribosomes. Lack of Pub1 decreased efficiency of nonsense readthrough at a majority but not all tetranucleotide stop signals. This distinguishes Pub1 from most other known binding partners of the release factors which were shown to modulate readthrough of all nonsense codons uniformly. The obtained data show that Pub1 can act as an accessory translation factor involved in fine‐tuning of translation termination.

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Details

Published: May 29, 2017
Vol/Issue: 284(12)
Pages: 1914-1930
License: View

Authors

V

Valery N. Urakov

Federal Research Center ‘Fundamentals of Biotechnology’ of the Russian Academy of Sciences Bach Institute of Biochemistry Moscow Russia

O

Olga V. Mitkevich

Federal Research Center ‘Fundamentals of Biotechnology’ of the Russian Academy of Sciences Bach Institute of Biochemistry Moscow Russia

I

Irina V. Safenkova

Federal Research Center ‘Fundamentals of Biotechnology’ of the Russian Academy of Sciences Bach Institute of Biochemistry Moscow Russia

M

Michael D. Ter‐Avanesyan

Federal Research Center ‘Fundamentals of Biotechnology’ of the Russian Academy of Sciences Bach Institute of Biochemistry Moscow Russia

Funding

Russian Foundation for Basic Research Award: #14‐04‐00073 and 17‐04‐00032

Russian Academy of Sciences

Cite This Article

Valery N. Urakov, Olga V. Mitkevich, Irina V. Safenkova, et al. (2017). Ribosome‐bound Pub1 modulates stop codon decoding during translation termination in yeast. The FEBS Journal, 284(12), 1914-1930. https://doi.org/10.1111/febs.14099

Ribosome‐bound Pub1 modulates stop codon decoding during translation termination in yeast

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