Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in
                    Plutella xylostella

K. Etebari; M. H. Afrad; B. Tang; R. Silva; M. J. Furlong; S. Asgari

doi:10.1111/imb.12387

journal article Mar 24, 2018

Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in Plutella xylostella

K. Etebari M. H. Afrad B. Tang R. Silva

M. J. Furlong S. Asgari

Insect Molecular Biology Vol. 27 No. 4 pp. 478-491 · Wiley

View at Publisher Save 10.1111/imb.12387

Abstract

Abstract

The diamondback moth,
Plutella xylostella
, has developed extremely high levels of resistance to chlorantraniliprole and other classes of insecticides in the field. As microRNAs (miRNAs) play important roles in various biological processes through gene regulation, we examined the miRNA profile of
P. xylostella
in response to chlorantraniliprole exposure. RNA sequencing analysis showed that insecticide treatment caused significant changes in the abundance of some miRNAs. Increasing exposure time and insecticide concentration induced more dysregulated miRNAs in
P. xylostella
larvae. We also screened potential target genes for some of the differentially expressed miRNAs (such as miR‐2b‐3p, miR‐14b‐5p and let‐7‐5p), which may play important roles in insecticide resistance development. Exposure of
P. xylostella
larvae to chlorantraniliprole caused considerable overexpression in the transcript levels of potential target genes
cytochrome P450 9f2
(
CYP9F2
) and
307a1
(
CYP307a1
). Application of miR‐2b‐3p and miR‐14b‐5p mimics significantly suppressed the relative transcript levels of
CYP9F2
and
CYP307a1
, respectively, in a
P. xylostella
cell line. Furthermore, enrichment of
P. xylostella
diet with miR‐2b‐3p mimics significantly increased mortality in deltamethrin‐resistant larvae when exposed to deltamethrin. The results suggest that miR‐2b‐3p may suppress
CYP9F2
transcript levels in
P. xylostella
and consequently inhibit larval detoxification pathways. The findings provide an insight into possible role of miRNAs in regulation of metabolic resistance of insects to insecticides.

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Details

Published: Mar 24, 2018
Vol/Issue: 27(4)
Pages: 478-491
License: View

Authors

K

K. Etebari