Differentially expressed cellular genes following HBV: potential targets of anti‐HBV drugs?

J. Yang; X. C. Bo; J. Yao; N. M. Yang; S. Q. Wang

doi:10.1111/j.1365-2893.2005.00611.x

journal article Jun 28, 2005

Differentially expressed cellular genes following HBV: potential targets of anti‐HBV drugs?

J. Yang

X. C. Bo J. Yao N. M. Yang S. Q. Wang

Journal of Viral Hepatitis Vol. 12 No. 4 pp. 357-363 · Wiley

View at Publisher Save 10.1111/j.1365-2893.2005.00611.x

Abstract

Summary. The aim of the study was to screen for cellular genes that are differentially expressed following hepatitis B virus (HBV) infection, in an attempt to identify potential targets of anti‐HBV drugs. An oligonucleotide microarray containing 231 virus‐infection‐associated genes was prepared. Differential gene expression in HepG2.2.15 cells compared to control with HepG2 cells was analysed by this in‐house microarray. The change in gene expression in HepG2.2.15 cells treated by lamivudine on days 4 and 8 after exposure was also studied. Semi‐quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) was used to comfirm the differentially expressed genes induced by HBV and lamivudine. There were 31 upregulated and four downregulated genes in HepG2.2.15 cells compared with the HepG2 control cells. Eleven genes were consistently altered by lamivudine at both time points. Of the 31 genes that were upregulated in HepG2.2.15 cells, there were seven genes which were downregulated by lamivudine. Of the four downregulated genes, there was one gene which was upregulated by lamivudine. Of the differentially expressed genes induced by HBV and lamivudine, the expression of five genes was confirmed by semi‐quantitative RT‐PCR. These results shed new light on the effects of HBV and lamivudine on cellular gene expression. Differentially expressed genes induced by HBV and lamivudine could potentially become new anti‐HBV drug targets in novel therapies.

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Citations

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References

Details

Published: Jun 28, 2005
Vol/Issue: 12(4)
Pages: 357-363
License: View

Authors

D-Wave Systems, 3033 Beta Avenue, Burnaby, BC V5G 4M9, Canada.

Cite This Article

J. Yang, X. C. Bo, J. Yao, et al. (2005). Differentially expressed cellular genes following HBV: potential targets of anti‐HBV drugs?. Journal of Viral Hepatitis, 12(4), 357-363. https://doi.org/10.1111/j.1365-2893.2005.00611.x

Differentially expressed cellular genes following HBV: potential targets of anti‐HBV drugs?

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