Epigenetic and inflammatory events in experimental periodontitis following systemic microbial challenge

Daniela B. Palioto; Livia S. Finoti; Denis F. Kinane; Manjunatha Benakanakere

doi:10.1111/jcpe.13151

journal article Jun 25, 2019

Epigenetic and inflammatory events in experimental periodontitis following systemic microbial challenge

Daniela B. Palioto Livia S. Finoti Denis F. Kinane Manjunatha Benakanakere

Journal of Clinical Periodontology Vol. 46 No. 8 pp. 819-829 · Wiley

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Abstract

AbstractAimThe purpose of this study was to determine inflammatory and epigenetic features following induction of oral and gut dysbiosis in experimental periodontitis in order to examine the interplay between oral and systemic infection.Materials and MethodsPeriodontitis was induced in 6‐ to 8‐week‐old C57BL/6 mice by (a) Ligature placement (Lig group) (oral challenge); (b) P. gingivalis gavage (Pg group) (systemic challenge); and (c) the combination of the two models oral and systemic challenge (Pg + Lig). The duration of the experiment was 60 days, and the animals were then sacrificed for analyses. Alveolar bone loss was assessed, and a multiplex immunoassay was performed. Maxillae and gut tissues were immunostained for DNMT3b (de novo methylation marker), B and T lymphocyte attenuator (BTLA) and IL‐18R1 (inflammation markers).ResultsPg and Pg + Lig groups exhibited higher bone loss when compared to Sham. BAFF, VEGF, RANKL, RANTES and IP‐10 were significantly higher with Pg gavage. Likewise, DNMT3b was overexpressed in both gut and maxilla after the Pg administration. The same pattern was observed for BTLA and IL‐18R1 in gut tissues.ConclusionsThe systemic microbial challenge either alone or in combination with local challenge leads to distinct patterns of inflammatory and epigenetic features when compared to simply locally induced experimental periodontitis.

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References

Details

Published: Jun 25, 2019
Vol/Issue: 46(8)
Pages: 819-829
License: View

Authors

D

Daniela B. Palioto

Department of Periodontics, School of Dental Medicine University of Pennsylvania Philadelphia Pennsylvania; Department of OMS and Periodontology University of São Paulo ‐ School of Dentistry of Ribeirão Preto Ribeirão Preto Brazil

L

Livia S. Finoti

Department of Periodontics, School of Dental Medicine University of Pennsylvania Philadelphia Pennsylvania

D

Denis F. Kinane

Division of Periodontology, School of Dental Medicine University of Geneva Geneva Switzerland

M

Manjunatha Benakanakere

Department of Periodontics, School of Dental Medicine University of Pennsylvania Philadelphia Pennsylvania

Funding

National Institute of Dental and Craniofacial Research Award: R01DE024160

Fundação Amazônia Paraense de Amparo á Pesquisa Award: 2016/21471‐0

Cite This Article

Daniela B. Palioto, Livia S. Finoti, Denis F. Kinane, et al. (2019). Epigenetic and inflammatory events in experimental periodontitis following systemic microbial challenge. Journal of Clinical Periodontology, 46(8), 819-829. https://doi.org/10.1111/jcpe.13151

Epigenetic and inflammatory events in experimental periodontitis following systemic microbial challenge

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