Minimal risk of tilapia lake virus transmission via frozen tilapia fillets

Worrayanee Thammatorn; Pattarasuda Rawiwan; Win Surachetpong

doi:10.1111/jfd.12924

journal article Nov 23, 2018

Minimal risk of tilapia lake virus transmission via frozen tilapia fillets

Worrayanee Thammatorn Pattarasuda Rawiwan

Win Surachetpong

Journal of Fish Diseases Vol. 42 No. 1 pp. 3-9 · Wiley

View at Publisher Save 10.1111/jfd.12924

Abstract

AbstractRecent outbreaks of a novel tilapia lake virus (TiLV) have raised concerns regarding the international spread of TiLV in frozen tilapia products. This study investigated the potential risks of frozen tilapia fillet as a source of TiLV transmission. It revealed that TiLV genomic RNA could be detected in tilapia fillet and the virus isolated from non‐frozen and frozen fillets with clinical TiLV infection stored up to 28 days caused a cytopathic effect (CPE) formation in the susceptible cell line in vitro. However, frozen fillets from clinical TiLV infection stored for 90 and 120 days did not cause CPE in the susceptible cell line. Similarly, CPE was not observed in TiLV isolated from subclinically TiLV‐infected fish fillets. In addition, in vivo bioassay revealed that despite the presence of TiLV isolated from subclinically TiLV‐infected fillet stored at −20°C for 14 days, there was no evidence of TiLV disease in naïve red hybrid tilapia based on the absence of clinical signs and mortality and without the detection of TiLV genomic RNA using reverse transcription–quantitative polymerase chain reaction assay. Collectively, these findings suggested minimal risk of transmission of TiLV via frozen tilapia fillets.

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Metrics

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Citations

25

References

Details

Published: Nov 23, 2018
Vol/Issue: 42(1)
Pages: 3-9
License: View

Authors

W

Worrayanee Thammatorn

Department of Veterinary Microbiology and Immunology Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand

P

Pattarasuda Rawiwan

Department of Veterinary Microbiology and Immunology Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand; Center for Advanced Studies for Agriculture and Food Institute for Advanced Studies Kasetsart University Bangkok Thailand

W

Win Surachetpong

Department of Veterinary Microbiology and Immunology Faculty of Veterinary Medicine Kasetsart University Bangkok Thailand; Center for Advanced Studies for Agriculture and Food Institute for Advanced Studies Kasetsart University Bangkok Thailand

Funding

The Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University

The Higher Education Research Promotion and National Research University Project of Thailand

Office of the Higher Education Commission, Ministry of Education, Thailand

Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand

Cite This Article

Worrayanee Thammatorn, Pattarasuda Rawiwan, Win Surachetpong (2018). Minimal risk of tilapia lake virus transmission via frozen tilapia fillets. Journal of Fish Diseases, 42(1), 3-9. https://doi.org/10.1111/jfd.12924

Minimal risk of tilapia lake virus transmission via frozen tilapia fillets

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