Advances in 3D cell culture technologies enabling tissue‐like structures to be created in vitro

Eleanor Knight; Stefan Przyborski

doi:10.1111/joa.12257

journal article Open Access Nov 20, 2014

Advances in 3D cell culture technologies enabling tissue‐like structures to be created in vitro

Eleanor Knight Stefan Przyborski

Journal of Anatomy Vol. 227 No. 6 pp. 746-756 · Wiley

View at Publisher Save 10.1111/joa.12257

Abstract

AbstractResearch in mammalian cell biology often relies on developing in vitro models to enable the growth of cells in the laboratory to investigate a specific biological mechanism or process under different test conditions. The quality of such models and how they represent the behavior of cells in real tissues plays a critical role in the value of the data produced and how it is used. It is particularly important to recognize how the structure of a cell influences its function and how co‐culture models can be used to more closely represent the structure of real tissue. In recent years, technologies have been developed to enhance the way in which researchers can grow cells and more readily create tissue‐like structures. Here we identify the limitations of culturing mammalian cells by conventional methods on two‐dimensional (2D) substrates and review the popular approaches currently available that enable the development of three‐dimensional (3D) tissue models in vitro. There are now many ways in which the growth environment for cultured cells can be altered to encourage 3D cell growth. Approaches to 3D culture can be broadly categorized into scaffold‐free or scaffold‐based culture systems, with scaffolds made from either natural or synthetic materials. There is no one particular solution that currently satisfies all requirements and researchers must select the appropriate method in line with their needs. Using such technology in conjunction with other modern resources in cell biology (e.g. human stem cells) will provide new opportunities to create robust human tissue mimetics for use in basic research and drug discovery. Application of such models will contribute to advancing basic research, increasing the predictive accuracy of compounds, and reducing animal usage in biomedical science.

Topics

No keywords indexed for this article. Browse by subject →

References

94

[1]

10.1006/excr.1996.0340

[2]

10.1208/s12248-013-9525-z

[3]

10.4161/cc.25163

[4]

10.1007/978-1-62703-128-8_32

[5]

10.1016/j.biomaterials.2003.10.045

[6]

10.1002/term.187

[7]

10.1387/ijdb.052072hb

[8]

10.1242/jcs.079509

[9]

10.1016/j.biomaterials.2007.01.004

[10]

10.1007/s10616-006-9001-z

[11]

10.1016/j.biomaterials.2005.04.003

[12]

10.1016/s1359-6446(02)02273-0

[13]

10.1111/j.1469-7580.2007.00778.x

[14]

10.1016/j.bbrc.2007.01.105

[15]

10.1039/b707499a

[16]

10.1002/btpr.139

[17]

10.3109/00498254.2012.675093

[18]

10.4161/org.5.2.8321

[19]

10.1039/b603211g

[20]

10.1016/j.biomaterials.2013.11.038

[21]

10.1016/j.biomaterials.2013.07.035

[22]

10.1038/srep04414

[23]

10.1016/s0955-0674(02)00364-2

[24]

10.1182/blood.v93.5.1658

[25]

10.1016/j.tiv.2008.07.003

[26]

Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures

Jayanta Debnath, Senthil K. Muthuswamy, Joan S. Brugge

Methods 10.1016/s1046-2023(03)00032-x

[27]

10.1089/107632703764664783

[28]

10.1096/fj.07-9277com

[29]

10.1371/journal.pone.0021496

[30]

Matrix Elasticity Directs Stem Cell Lineage Specification

Adam J. Engler, Shamik Sen, H. Lee Sweeney et al.

Cell 10.1016/j.cell.2006.06.044

[31]

10.1038/sj.bjc.6605610

[32]

10.1016/j.molmed.2008.06.001

[33]

10.1016/j.biomaterials.2007.01.021

[34]

10.1038/nmeth1085

[35]

Spheroid-based drug screen: considerations and practical approach

Juergen Friedrich, Claudia Seidel, Reinhard Ebner et al.

Nature Protocols 10.1038/nprot.2008.226

[36]

10.1089/ten.2006.12.2215

[37]

10.1073/pnas.0703723104

[38]

Biodegradable synthetic polymers for tissue engineering

PA Gunatillake

European Cells and Materials 10.22203/ecm.v005a01

[39]

10.1089/ten.tea.2010.0273

[40]

10.1016/j.bbrc.2003.12.135

[41]

10.1016/j.jbbm.2004.12.001

[42]

10.1002/marc.201300709

[43]

10.1089/ten.tea.2009.0060

[44]

10.1089/ten.2004.10.1467

[45]

Multicellular tumor spheroids: An underestimated tool is catching up again

Franziska Hirschhaeuser, Heike Menne, Claudia Dittfeld et al.

Journal of Biotechnology 10.1016/j.jbiotec.2010.01.012

[46]

10.1016/j.biomaterials.2008.04.040

[47]

10.1016/0955-0674(95)80071-9

[48]

10.1016/j.jconrel.2004.04.009

[49]

10.1016/j.semcancer.2005.05.004

[50]

10.1007/978-1-60761-984-0_20

Showing 50 of 94 references

Cited By

453

Self-sustaining media-reconstituted GelMA bioink supports printable hematopoietic cultures

Noah S. Pereira, Jacob Quint · 2026

Biomaterials Science

In Vitro Disease Models of the Endocrine Pancreas

Marko Milojević, Jan Rožanc · 2021

Biomedicines

In situ construction of a self-assembled AIE probe for tumor hypoxia imaging

Tianhao Xue, Kuanchun Shao · 2020

Nanoscale

Metrics

453

Citations

94

References

Details

Published: Nov 20, 2014
Vol/Issue: 227(6)
Pages: 746-756
License: View

Authors

E

Eleanor Knight

School of Biological and Biomedical Science Durham University Durham UK

S

Stefan Przyborski

School of Biological and Biomedical Science Durham University Durham UK

Funding

Biotechnology and Biological Sciences Research Council

Medical Research Council

Cite This Article

Eleanor Knight, Stefan Przyborski (2014). Advances in 3D cell culture technologies enabling tissue‐like structures to be created in vitro. Journal of Anatomy, 227(6), 746-756. https://doi.org/10.1111/joa.12257

Advances in 3D cell culture technologies enabling tissue‐like structures to be created in vitro

You May Also Like