Lipidomic analysis can distinguish between two morphologically similar strains of Nannochloropsis oceanica

Shuang Li; Jilin Xu; Ying Jiang; Chengxu Zhou; Xuejun Yu; Yingying Zhong; Juanjuan Chen; Xiaojun Yan

doi:10.1111/jpy.12271

journal article Mar 03, 2015

Lipidomic analysis can distinguish between two morphologically similar strains of Nannochloropsis oceanica

Shuang Li

Jilin Xu Ying Jiang

Chengxu Zhou Xuejun Yu Yingying Zhong Juanjuan Chen

Xiaojun Yan

Journal of Phycology Vol. 51 No. 2 pp. 264-276 · Wiley

View at Publisher Save 10.1111/jpy.12271

Abstract

The two morphologically similar microalgae NMBluh014 and NMBluh‐X belong to two different strains of Nannochloropsis oceanica. They possess obviously different feeding effects on bivalves, but are indistinguishable by 18S rRNA and morphological features. In this work, lipidomic analysis followed by principal component analysis and orthogonal projections to latent structures discriminant analysis provided a clear distinction between these strains. Metabolites that definitively contribute to the classification were selected as potential biomarkers. The most important difference in polar lipids were sulfoquinovosyldiacylglycerol (containing 18:1/16:0 and 18:3/16:0) and monogalactosyldiacylglycerol (containing 18:3/16:3 and 20:5/14:0), which were detected only in NMBluh‐X. Additionally, an exhaustive qualitative and quantitative profiling of the neutral lipid triacylglycerol (TAG) in the two strains was carried out. The predominant species of TAG containing 16:1/16:1/16:1 acyl groups was detected only in NMBluh‐X with a content of ~93.67 ± 11.85 nmol · mg−1 dry algae at the onset of stationary phase. Meanwhile, TAG containing 16:0/16:0/16:0 was the main TAG in NMBluh014 with a content of 40.25 ± 3.92 nmol · mg−1. These results provided the most straightforward evidence for differentiating the two species. The metabolomic profiling indicated that NMBluh‐X underwent significant chemical and physiological changes during the growth process, whereas NMBluh014 did not show such noticeable time‐dependent metabolite change. This study is the first using Ultra Performance Liquid Chromatography coupled with Electrospray ionization‐Quadrupole‐Time of Flight Mass Spectrometry (UPLC‐Q‐TOF‐MS) for lipidomic profiling with multivariate statistical analysis to explore lipidomic differences of plesiomorphous microalgae. Our results demonstrate that lipidomic profiling is a valid chemotaxonomic tool in the study of microalgal systematics.

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References

Details

Published: Mar 03, 2015
Vol/Issue: 51(2)
Pages: 264-276
License: View

Authors

S

Shuang Li

Key Laboratory of Applied Marine Biotechnology Ningbo University Chinese Ministry of Education Ningbo Zhejiang 315211 China; Ningbo Entry‐Exit Inspection and Quarantine Bureau Technology Center of the People's Republic of China Ningbo Zhejiang 315211 China; School of Marine Sciences Ningbo University Ningbo Zhejiang 315211 China

J

Jilin Xu

Key Laboratory of Applied Marine Biotechnology Ningbo University Chinese Ministry of Education Ningbo Zhejiang 315211 China

Y

Ying Jiang

School of Marine Sciences Ningbo University Ningbo Zhejiang 315211 China

C

Chengxu Zhou

School of Marine Sciences Ningbo University Ningbo Zhejiang 315211 China

X

Xuejun Yu

Ningbo Entry‐Exit Inspection and Quarantine Bureau Technology Center of the People's Republic of China Ningbo Zhejiang 315211 China

Y

Yingying Zhong

Ningbo Entry‐Exit Inspection and Quarantine Bureau Technology Center of the People's Republic of China Ningbo Zhejiang 315211 China

J

Juanjuan Chen

Key Laboratory of Applied Marine Biotechnology Ningbo University Chinese Ministry of Education Ningbo Zhejiang 315211 China

X

Xiaojun Yan

School of Marine Sciences Ningbo University Ningbo Zhejiang 315211 China

Funding

National Natural Science Foundation of China Award: 31172448

Zhejiang Natural Science Foundation Award: LY12D06001

National Sparking Plan Project of China Award: 2014GA720002

Project of Ministry of Education Award: 20133305130001

Ningbo Science and Technology Research Projects Award: 2013C10014

Zhejiang Marine Biotechnology Innovation Team Award: 2012R10029

Ningbo Marine Algae Biotechnology Team Award: 2011B81007

Zhejiang Public Welfare Project Award: 2014C32081

Quality Supervision, Inspection and Quarantine of Science and Technology Plan Projects Award: 2013IK171

Ningbo Entry-Exit Inspection and Quarantine Bureau of Science and Technology Projects Award: K14-2013

Analysis of Science and Technology Plan Projects Award: 2012C37072

Ningbo Natural Science Fund Award: 2012A610149

Cite This Article

Shuang Li, Jilin Xu, Ying Jiang, et al. (2015). Lipidomic analysis can distinguish between two morphologically similar strains of Nannochloropsis oceanica. Journal of Phycology, 51(2), 264-276. https://doi.org/10.1111/jpy.12271

Lipidomic analysis can distinguish between two morphologically similar strains of Nannochloropsis oceanica

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