Co‐suppression in Pyropia yezoensis (Rhodophyta) Reveals the Role of PyLHCI in Light Harvesting and Generation Switch

Zhenbing Zheng; Bangxiang He; Xiujun Xie; Guangce Wang

doi:10.1111/jpy.13073

journal article Nov 24, 2020

Co‐suppression in Pyropia yezoensis (Rhodophyta) Reveals the Role of PyLHCI in Light Harvesting and Generation Switch

Zhenbing Zheng Bangxiang He Xiujun Xie Guangce Wang

Journal of Phycology Vol. 57 No. 1 pp. 160-171 · Wiley

View at Publisher Save 10.1111/jpy.13073

Abstract

The red macroalga Pyropia yezoensis is an economically important seaweed widely cultured in Asian countries and is a model organism for molecular biological and commercial research. This species is unique in that it utilizes both phycobilisomes and transmembrane light‐harvesting proteins as its antenna system. Here, one of the genes of P. yezoensis (PyLHCI) was selected for introduction into its genome to overexpress PyLHCI. However, the co‐suppression phenomenon occurred. This is the first documentation of co‐suppression in algae, in which it exhibits a different mechanism from that in higher plants. The transformant (T1) was demonstrated to have higher phycobilisomes and lower LHC binding pigments, resulting in a redder color, higher sensitivity to salt stress, smaller in size, and slower growth rate than the wildtype (WT). The photosynthetic performances of T1 and WT showed similar characteristics; however, P700 reduction was slower in T1. Most importantly, T1 could release a high percentage of carpospores in young blades to switch generation during its life cycle, which was rarely seen in WT. The co‐suppression of PyLHCI revealed its key roles in light harvesting, stress resistance, and generation alternation (generation switch from gametophytes to sporophytes, and reproduction from asexual to sexual).

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Details

Published: Nov 24, 2020
Vol/Issue: 57(1)
Pages: 160-171
License: View

Authors

Z

Zhenbing Zheng

Key Laboratory of Experimental Marine Biology Center for Ocean Mega‐Science Institute of Oceanology Chinese Academy of Sciences Qingdao China

B

Bangxiang He

Key Laboratory of Experimental Marine Biology Center for Ocean Mega‐Science Institute of Oceanology Chinese Academy of Sciences Qingdao China

X

Xiujun Xie

Key Laboratory of Experimental Marine Biology Center for Ocean Mega‐Science Institute of Oceanology Chinese Academy of Sciences Qingdao China

G

Guangce Wang

Key Laboratory of Experimental Marine Biology Center for Ocean Mega‐Science Institute of Oceanology Chinese Academy of Sciences Qingdao China; Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao China

Funding

China Postdoctoral Science Foundation Award: 2019M652594

Cite This Article

Zhenbing Zheng, Bangxiang He, Xiujun Xie, et al. (2020). Co‐suppression in Pyropia yezoensis (Rhodophyta) Reveals the Role of PyLHCI in Light Harvesting and Generation Switch. Journal of Phycology, 57(1), 160-171. https://doi.org/10.1111/jpy.13073

Co‐suppression in Pyropia yezoensis (Rhodophyta) Reveals the Role of PyLHCI in Light Harvesting and Generation Switch

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