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journal article Jan 20, 2014

Inferring the contribution of sexual reproduction, migration and off‐season survival to the temporal maintenance of microbial populations: a case study on the wheat fungal pathogen Puccinia striiformis f.sp. tritici

Sajid Ali ORCID Pierre Gladieux Hidayatur Rahman Muhammad S. Saqib Muhammad Fiaz ORCID Habib Ahmad Marc Leconte Angélique Gautier Annemarie F. Justesen Mogens S. Hovmøller Jérôme Enjalbert Claude de Vallavieille‐Pope
Molecular Ecology Vol. 23 No. 3 pp. 603-617 · Wiley
View at Publisher Save 10.1111/mec.12629
Abstract
AbstractUnderstanding the mode of temporal maintenance of plant pathogens is an important domain of microbial ecology research. Due to the inconspicuous nature of microbes, their temporal maintenance cannot be studied directly through tracking individuals and their progeny. Here, we suggest a series of population genetic analyses on molecular marker variation in temporally spaced samples to infer about the relative contribution of sexual reproduction, off‐season survival and migration to the temporal maintenance of pathogen populations. We used the proposed approach to investigate the temporal maintenance of wheat yellow rust pathogen, Puccinia striiformis f.sp. tritici (PST), in the Himalayan region of Pakistan. Multilocus microsatellite genotyping of PST isolates revealed high genotypic diversity and recombinant population structure across all locations, confirming the existence of sexual reproduction in this region. The genotypes were assigned to four genetic groups, revealing a clear differentiation between zones with and without Berberis spp., the alternate host of PST, with an additional subdivision within the Berberis zone. The lack of any differentiation between samples across two sampling years, and the very infrequent resampling of multilocus genotypes over years at a given location was consistent with limited over‐year clonal survival, and a limited genetic drift. The off‐season oversummering population in the Berberis zone, likely to be maintained locally, served as a source of migrants contributing to the temporal maintenance in the non‐Berberis zone. Our study hence demonstrated the contribution of both sexual recombination and off‐season oversummering survival to the temporal maintenance of the pathogen. These new insights into the population biology of PST highlight the general usefulness of the analytical approach proposed.
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Cited By
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Hanna Susi, Jeremy J. Burdon · 2020

PLOS Pathogens
Metrics
87
Citations
69
References
Details
Published
Jan 20, 2014
Vol/Issue
23(3)
Pages
603-617
License
View
Authors
S
Sajid Ali

INRA UR 1290 BIOGER‐CPP BP01 78850 Thiverval‐Grignon France; Institute of Biotechnology and Genetic Engineering The University of Agriculture Peshawar 25000 Pakistan; Department of Agroecology Aarhus University Flakkebjerg DK‐4200 Slagelse Denmark

P
Pierre Gladieux

Department of Plant and Microbial Biology University of California Berkeley CA 94720‐3102 USA; Ecologie, Systématique et Evolution UMR8079 Univ Paris‐Sud 91405 Orsay France; Ecologie, Systématique et Evolution UMR8079 CNRS 91405 Orsay France

H
Hidayatur Rahman

Department of Plant Breeding and Genetics The University of Agriculture Peshawar 25000 Pakistan

M
Muhammad S. Saqib

Department of Plant Breeding and Genetics The University of Agriculture Peshawar 25000 Pakistan

M
Muhammad Fiaz

Hazara University Mansehra 21000 Pakistan

H
Habib Ahmad

Hazara University Mansehra 21000 Pakistan

M
Marc Leconte

INRA UR 1290 BIOGER‐CPP BP01 78850 Thiverval‐Grignon France

A
Angélique Gautier

INRA UR 1290 BIOGER‐CPP BP01 78850 Thiverval‐Grignon France

A
Annemarie F. Justesen

Department of Agroecology Aarhus University Flakkebjerg DK‐4200 Slagelse Denmark

M
Mogens S. Hovmøller

Department of Agroecology Aarhus University Flakkebjerg DK‐4200 Slagelse Denmark

J
Jérôme Enjalbert

INRA UMR 320 Génétique Végétale Ferme du Moulon 91190 Gif sur Yvette France

C
Claude de Vallavieille‐Pope

INRA UR 1290 BIOGER‐CPP BP01 78850 Thiverval‐Grignon France

Funding
Marie Curie Grant Award: PIOF-GA-2010-273086 GEDiNdis
European Integrated Project BIOEXPLOIT Award: FOOD-CT-2005-513959
French ANR Project (EMERFUNDIS) Award: ANR 07-BDIV-003
Cite This Article
Sajid Ali, Pierre Gladieux, Hidayatur Rahman, et al. (2014). Inferring the contribution of sexual reproduction, migration and off‐season survival to the temporal maintenance of microbial populations: a case study on the wheat fungal pathogen Puccinia striiformis f.sp. tritici. Molecular Ecology, 23(3), 603-617. https://doi.org/10.1111/mec.12629
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