Characterization of the multiple molecular mechanisms underlying RsaL control of phenazine‐1‐carboxylic acid biosynthesis in the rhizosphere bacterium Pseudomonas aeruginosa PA1201

Shuang Sun; Bo Chen; Zi‐Jing Jin; Lian Zhou; Yun‐Ling Fang; Chitti Thawai; Giordano Rampioni; Ya‐Wen He

doi:10.1111/mmi.13671

journal article Apr 04, 2017

Characterization of the multiple molecular mechanisms underlying RsaL control of phenazine‐1‐carboxylic acid biosynthesis in the rhizosphere bacterium Pseudomonas aeruginosa PA1201

Shuang Sun

Bo Chen

Zi‐Jing Jin Lian Zhou

Yun‐Ling Fang Chitti Thawai Giordano Rampioni Ya‐Wen He

Molecular Microbiology Vol. 104 No. 6 pp. 931-947 · Wiley

View at Publisher Save 10.1111/mmi.13671

Abstract

SummaryPhenazines are important secondary metabolites that have been found to affect a broad spectrum of organisms. Two almost identical gene clusters phz1 and phz2 are responsible for phenazines biosynthesis in the rhizobacterium Pseudomonas aeruginosa PA1201. Here, we show that the transcriptional regulator RsaL is a potent repressor of phenazine‐1‐carboxylic acid (PCA) biosynthesis. RsaL negatively regulates phz1 expression and positively regulates phz2 expression via multiple mechanisms. First, RsaL binds to a 25‐bp DNA region within the phz1 promoter to directly repress phz1 expression. Second, RsaL indirectly regulates the expression of both phz clusters by decreasing the activity of the las and pqs quorum sensing (QS) systems, and by promoting the rhl QS system. Finally, RsaL represses phz1 expression through the downstream transcriptional regulator CdpR. RsaL directly binds to the promoter region of cdpR to positively regulate its expression, and subsequently CdpR regulates phz1 expression in a negative manner. We also show that RsaL represents a new mechanism for the turnover of the QS signal molecule N‐3‐oxododecanoyl‐homoserine lactone (3‐oxo‐C12‐HSL). Overall, this study elucidates RsaL control of phenazines biosynthesis and indicates that a PA1201 strain harboring deletions in both the rsaL and cdpR genes could be used to improve the industrial production of PCA.

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References

Details

Published: Apr 04, 2017
Vol/Issue: 104(6)
Pages: 931-947
License: View

Authors

S

Shuang Sun

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

B

Bo Chen

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

Z

Zi‐Jing Jin

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

L

Lian Zhou

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

Y

Yun‐Ling Fang

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

C

Chitti Thawai

Department of Biology, Faculty of Science King Mongkut's Institute of Technology Ladkrabang Bangkok Thailand

G

Giordano Rampioni

Department of Science University Roma Tre Rome Italy

Y

Ya‐Wen He

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

Funding

Shanghai Municipal Education Commission Award: GM0800004

Cite This Article

Shuang Sun, Bo Chen, Zi‐Jing Jin, et al. (2017). Characterization of the multiple molecular mechanisms underlying RsaL control of phenazine‐1‐carboxylic acid biosynthesis in the rhizosphere bacterium Pseudomonas aeruginosa PA1201. Molecular Microbiology, 104(6), 931-947. https://doi.org/10.1111/mmi.13671

Characterization of the multiple molecular mechanisms underlying RsaL control of phenazine‐1‐carboxylic acid biosynthesis in the rhizosphere bacterium Pseudomonas aeruginosa PA1201

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