Temporal patterns of orchid mycorrhizal fungi in meadows and forests as revealed by 454 pyrosequencing

Jane Oja; Petr Kohout; Leho Tedersoo; Tiiu Kull; Urmas Kõljalg

doi:10.1111/nph.13223

journal article Dec 24, 2014

Temporal patterns of orchid mycorrhizal fungi in meadows and forests as revealed by 454 pyrosequencing

Jane Oja Petr Kohout

Leho Tedersoo

Tiiu Kull Urmas Kõljalg

New Phytologist Vol. 205 No. 4 pp. 1608-1618 · Wiley

View at Publisher Save 10.1111/nph.13223

Abstract

Summary

Orchid mycorrhizal (OrM) symbionts play a key role in the growth of orchids, but the temporal variation and habitat partitioning of these fungi in roots and soil remain unclear.

Temporal changes in root and rhizosphere fungal communities of Cypripedium calceolus, Neottia ovata and Orchis militaris were studied in meadow and forest habitats over the vegetation period by using 454 pyrosequencing of the full internal transcribed spacer (ITS) region.

The community of typical OrM symbionts differed by plant species and habitats. The root fungal community of N. ovata changed significantly in time, but this was not observed in C. calceolus and O. militaris. The rhizosphere community included a low proportion of OrM symbionts that exhibited a slight temporal turnover in meadow habitats but not in forests. Habitat differences in OrM and all fungal associates are largely attributable to the greater proportion of ectomycorrhizal fungi in forests.

Temporal changes in OrM fungal communities in roots of certain species indicate selection of suitable fungal species by plants. It remains to be elucidated whether these shifts depend on functional differences inside roots, seasonality, climate or succession.

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Details

Published: Dec 24, 2014
Vol/Issue: 205(4)
Pages: 1608-1618
License: View

Authors

J

Jane Oja

Institute of Ecology and Earth Sciences University of Tartu 14A Ravila 50411 Tartu Estonia

P

Petr Kohout

Institute of Ecology and Earth Sciences University of Tartu 14A Ravila 50411 Tartu Estonia; Institute of Botany Academy of Sciences of the Czech Republic CZ‐252 43 Průhonice Czech Republic

L

Leho Tedersoo

Institute of Ecology and Earth Sciences University of Tartu 14A Ravila 50411 Tartu Estonia

T

Tiiu Kull

Institute of Agricultural and Environmental Sciences Estonian University of Life Sciences 5 Kreutzwaldi 51014 Tartu Estonia

U

Urmas Kõljalg

Institute of Ecology and Earth Sciences University of Tartu 14A Ravila 50411 Tartu Estonia

Cite This Article

Jane Oja, Petr Kohout, Leho Tedersoo, et al. (2014). Temporal patterns of orchid mycorrhizal fungi in meadows and forests as revealed by 454 pyrosequencing. New Phytologist, 205(4), 1608-1618. https://doi.org/10.1111/nph.13223

Temporal patterns of orchid mycorrhizal fungi in meadows and forests as revealed by 454 pyrosequencing

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