The transcription factor MYB115 contributes to the regulation of proanthocyanidin biosynthesis and enhances fungal resistance in poplar

Lijun Wang; Lingyu Ran; Yisu Hou; Qiaoyan Tian; Chaofeng Li; Rui Liu; Di Fan; Keming Luo

doi:10.1111/nph.14569

journal article Apr 26, 2017

The transcription factor MYB115 contributes to the regulation of proanthocyanidin biosynthesis and enhances fungal resistance in poplar

Lijun Wang

Lingyu Ran Yisu Hou Qiaoyan Tian Chaofeng Li

Rui Liu

Di Fan

Keming Luo

New Phytologist Vol. 215 No. 1 pp. 351-367 · Wiley

View at Publisher Save 10.1111/nph.14569

Abstract

SummaryProanthocyanidins (PAs) are major defense phenolic compounds in the leaves of poplar (Populusspp.) in response to abiotic and biotic stresses. Transcriptional regulation ofPAbiosynthetic genes by the MYB‐basic helix–loop–helix (bHLH)‐WD40 complexes in poplar is not still fully understood.Here, an Arabidopsis TT2‐like geneMYB115was isolated fromPopulus tomentosaand characterized by various molecular, genetic and biochemical approaches.MYB115restored PA productions in the seed coat of the Arabidopsistt2mutant. Overexpression ofMYB115in poplar activated expression ofPAbiosynthetic genes, resulting in a significant increase inPAconcentrations. By contrast, theCRISPR/Cas9‐generatedmyb115mutant exhibited reducedPAcontent and decreased expression ofPAbiosynthetic genes.MYB115 directly activated the promoters ofPA‐specific structural genes.MYB115 interacted with poplarTT8. Coexpression ofMYB115,TT8and poplarTTG1significantly enhanced the expression ofANR1andLAR3. Additionally, transgenic plants overexpressingMYB115had increased resistance to the fungal pathogenDothiorella gregaria, whereasmyb115mutant exhibited greater sensitivity compared with wild‐type plants.Our data provide insight into the regulatory mechanisms controllingPAbiosynthesis byMYB115 in poplar, which could be effectively employed for metabolic engineering ofPAs to improve resistance to fungal pathogens.

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Details

Published: Apr 26, 2017
Vol/Issue: 215(1)
Pages: 351-367
License: View

Authors

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Lijun Wang