Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae

Eltayb Abdellatef; Torsten Will; Aline Koch; Jafargholi Imani; Andreas Vilcinskas; Karl‐Heinz Kogel

doi:10.1111/pbi.12322

journal article Jan 14, 2015

Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid Sitobion avenae

Eltayb Abdellatef Torsten Will Aline Koch Jafargholi Imani

Andreas Vilcinskas

Karl‐Heinz Kogel

Plant Biotechnology Journal Vol. 13 No. 6 pp. 849-857 · Wiley

View at Publisher Save 10.1111/pbi.12322

Abstract

SummaryAphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross‐linked by intermolecular disulphide bridges. We investigated the possibility of controlling aphid infestation by host‐induced gene silencing (HIGS) targeting shp expression in the grain aphid Sitobion avenae. When aphids were fed on transgenic barley expressing shp double‐stranded RNA (shp‐dsRNA), they produced significantly lower levels of shp mRNA compared to aphids feeding on wild‐type plants, suggesting that the transfer of inhibitory RNA from the plant to the insect was successful. shp expression remained low when aphids were transferred from transgenic plants and fed for 1 or 2 weeks, respectively, on wild‐type plants, confirming that silencing had a prolonged impact. Reduced shp expression correlated with a decline in growth, reproduction and survival rates. Remarkably, morphological and physiological aberrations such as winged adults and delayed maturation were maintained over seven aphid generations feeding on wild‐type plants. Targeting shp expression therefore appears to cause strong transgenerational effects on feeding, development and survival in S. avenae, suggesting that the HIGS technology has a realistic potential for the control of aphid pests in agriculture.

Topics

No keywords indexed for this article. Browse by subject →

References

71

[1]

10.1186/1472-6750-3-1

[2]

10.1016/j.ibmb.2003.11.004

[3]

Arnaud G. (1918)

[4]

10.1038/nature02874

[5]

10.1038/nbt1359

[6]

10.1046/j.1365-3040.2003.00963.x

[7]

10.1371/journal.pone.0046343

[8]

10.1079/9780851988771.0000

[9]

Blackman R.L. (2000)

[10]

10.1038/sj.hdy.6800863

[11]

10.1016/j.tig.2006.03.003

[12]

10.1016/s0960-9822(02)00666-8

[13]

10.1002/pmic.200800692

[14]

10.1021/pr100881q

[15]

10.1038/nrg3355

[16]

10.14411/eje.2000.052

[17]

10.1603/en10153

[18]

10.1104/pp.72.2.326

[19]

10.1016/j.jinsphys.2009.03.002

[20]

Dixon A.F.G. (1987)

[21]

10.1371/journal.pone.0065392

[22]

10.1002/ps.659

[23]

10.1038/sj.cdd.4402253

[24]

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

Andrew Fire, SiQun Xu, Mary K. Montgomery et al.

Nature 10.1038/35888

[25]

10.1603/0013-8746(2001)094[0761:spbbah]2.0.co;2

[26]

10.1093/jxb/ern181

[27]

10.1111/j.1440-169x.2006.00881.x

[28]

Helden M. (2000)

[29]

10.1242/jeb.046326

[30]

10.1016/j.jinsphys.2009.10.004

[31]

10.1111/j.1365-313x.2011.04620.x

[32]

Genome Sequence of the Pea Aphid Acyrthosiphon pisum

PLOS Biology 10.1371/journal.pbio.1000313

[33]

10.1186/1472-6750-7-63

[34]

Nonparametric Estimation from Incomplete Observations

E. L. Kaplan, Paul Meier

Journal of the American Statistical Association 1985 10.1080/01621459.1958.10501452

[35]

10.1111/pbi.12226

[36]

10.1073/pnas.1306373110

[37]

10.1073/pnas.1001945107

[38]

10.1038/nbt1091-963

[39]

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen

Methods 10.1006/meth.2001.1262

[40]

10.1038/nbt1352

[41]

10.1016/0022-1910(65)90119-8

[42]

10.1111/j.1469-185x.1999.tb00181.x

[43]

10.1371/journal.pone.0047431

[44]

10.1371/journal.pone.0062444

[45]

10.1673/031.006.3801

[46]

10.1073/pnas.0708958105

[47]

10.1105/tpc.110.077040

[48]

10.1094/mpmi-07-12-0172-fi

[49]

10.1371/journal.pone.0025709

[50]

10.1111/j.1570-7458.1994.tb01813.x

Showing 50 of 71 references

Cited By

120

The development and prospects of insect-resistant crops expressing double-strand RNAs

Wei Zhang, Zaihui Zhou · 2025

New Crops

Spray‐induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Lulu Qiao, Chi Lan · 2021

Plant Biotechnology Journal

RNAi-Based Functional Genomics in Hemiptera

Ritesh G. Jain, Karl E. Robinson · 2020

Insects

Functional Evaluation of Proteins in Watery and Gel Saliva of Aphids

Aart J. E. van Bel, Torsten Will · 2016

Frontiers in Plant Science

Metrics

120

Citations

71

References

Details

Published: Jan 14, 2015
Vol/Issue: 13(6)
Pages: 849-857
License: View

Authors

E

Eltayb Abdellatef