A fungal CFEM‐containing effector targets NPR1 regulator NIMIN2 to suppress plant immunity

Shengping Shang; Guangli Liu; Song Zhang; Rong Zhang; Guangyu Sun

doi:10.1111/pbi.14166

journal article Open Access Aug 19, 2023

A fungal CFEM‐containing effector targets NPR1 regulator NIMIN2 to suppress plant immunity

Shengping Shang

Plant Biotechnology Journal Vol. 22 No. 1 pp. 82-97 · Wiley

View at Publisher Save 10.1111/pbi.14166

Abstract

SummaryColletotrichum fructicola causes a broad range of plant diseases worldwide and secretes many candidate proteinous effectors during infection, but it remains largely unknown regarding their effects in conquering plant immunity. Here, we characterized a novel effector CfEC12 that is required for the virulence of C. fructicola. CfEC12 contains a CFEM domain and is highly expressed during the early stage of host infection. Overexpression of CfEC12 suppressed BAX‐triggered cell death, callose deposition and ROS burst in Nicotiana benthamiana. CfEC12 interacted with apple MdNIMIN2, a NIM1‐interacting (NIMIN) protein that putatively modulates NPR1 activity in response to SA signal. Transient expression and transgenic analyses showed that MdNIMIN2 was required for apple resistance to C. fructicola infection and rescued the defence reduction in NbNIMIN2‐silenced N. benthamiana, supporting a positive role in plant immunity. CfEC12 and MdNPR1 interacted with a common region of MdNIMIN2, indicating that CfEC12 suppresses the interaction between MdNIMIN2 and MdNPR1 by competitive target binding. In sum, we identified a fungal effector that targets the plant salicylic acid defence pathway to promote fungal infection.

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References

Details

Published: Aug 19, 2023
Vol/Issue: 22(1)
Pages: 82-97
License: View

Authors

S

Shengping Shang

State Key Laboratory of Crop Stress Biology in Arid Areas, Key Laboratory of Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Minishtry of Agriculture and Rural Affairs, and College of Plant Protection Northwest A&F University Yangling China

G

Guangli Liu

State Key Laboratory of Crop Stress Biology in Arid Areas, Key Laboratory of Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Minishtry of Agriculture and Rural Affairs, and College of Plant Protection Northwest A&F University Yangling China

S

Song Zhang

State Key Laboratory of Crop Stress Biology in Arid Areas, Key Laboratory of Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Minishtry of Agriculture and Rural Affairs, and College of Plant Protection Northwest A&F University Yangling China

R

Rong Zhang

State Key Laboratory of Crop Stress Biology in Arid Areas, Key Laboratory of Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Minishtry of Agriculture and Rural Affairs, and College of Plant Protection Northwest A&F University Yangling China

G

Guangyu Sun

State Key Laboratory of Crop Stress Biology in Arid Areas, Key Laboratory of Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Minishtry of Agriculture and Rural Affairs, and College of Plant Protection Northwest A&F University Yangling China

Funding

National Natural Science Foundation of China Award: 32070144;32072374

Cite This Article

Shengping Shang, Guangli Liu, Song Zhang, et al. (2023). A fungal CFEM‐containing effector targets NPR1 regulator NIMIN2 to suppress plant immunity. Plant Biotechnology Journal, 22(1), 82-97. https://doi.org/10.1111/pbi.14166

A fungal CFEM‐containing effector targets NPR1 regulator NIMIN2 to suppress plant immunity

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