UGT89AC1‐mediated quercetin glucosylation is induced upon herbivore damage and enhances Camellia sinensis resistance to insect feeding

AbstractQuercetin is a key flavonol in tea plants (Camellia sinensis (L.) O. Kuntze) with various health benefits, and it often occurs in the form of glucosides. The roles of quercetin and its glucosylated forms in plant defense are generally not well‐studied, and remain unknown in the defense of tea. Here, we found higher contents of quercetin glucosides and a decline of the aglucone upon Ectropis grisescens (E. grisescens) infestation of tea. Nine UGTs were strongly induced, among which UGT89AC1 exhibited the highest activity toward quercetin in vitro and in vivo. The mass of E. grisescens larvae that fed on plants with repressed UGT89AC1 or varieties with lower levels of UGT89AC1 was significantly lower than that of larvae fed on controls. Artificial diet supplemented with quercetin glucoside also reduced the larval growth rate, whereas artificial diet supplemented with free quercetin had no significant effect on larval growth. UGT89AC1 was located in both the cytoplasm and nucleus, and its expression was modulated by JA, JA‐ILE, and MeJA. These findings demonstrate that quercetin glucosylation serves a defensive role in tea against herbivory. Our results also provide novel insights into the ecological relevance of flavonoid glycosides under biotic stress in plants.

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Published: Oct 26, 2023
Vol/Issue: 47(2)
Pages: 682-697
License: View

Authors

T

Tingting Jing

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

W

Wenkai Du

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

X

Xiaona Qian

Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences Hangzhou China

K

KAI WANG

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

L

Linbao Luo

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

X

Xueying Zhang

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

Y

Yanni Deng

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

B

Bo Li

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

T

Ting Gao

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

M

Meilan Zhang

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

D

Deyin Guo

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

H

Hao Jiang

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

Y

Ying Liu

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

W

Wilfried Schwab

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China; Biotechnology of Natural Products, Technische Universität München Freising Germany

X

Xiaoling Sun

Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences Hangzhou China

C

Chuankui Song

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui China

Cite This Article

Tingting Jing, Wenkai Du, Xiaona Qian, et al. (2023). UGT89AC1‐mediated quercetin glucosylation is induced upon herbivore damage and enhances Camellia sinensis resistance to insect feeding. Plant, Cell & Environment, 47(2), 682-697. https://doi.org/10.1111/pce.14751

UGT89AC1‐mediated quercetin glucosylation is induced upon herbivore damage and enhances Camellia sinensis resistance to insect feeding

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