journal article Nov 09, 2025

Characterisation of the Pathogenic Colletotrichum gloeosporioides Causing Anthracnose of Tetrastigma hemsleyanum

Plant Pathology Vol. 75 No. 1 · Wiley
View at Publisher Save 10.1111/ppa.70090
Abstract
ABSTRACT

Tetrastigma hemsleyanum
, a herbaceous vine native to China, possesses significant therapeutic potential. However, its cultivation is increasingly threatened by anthracnose, a fungal disease that leads to substantial plant mortality. Therefore, evaluating the resistance of
T. hemsleyanum
to anthracnose is of critical importance. In this study, two members of the
Colletotrichum gloeosporioides
species complex—
C. gloeosporioides
and
C
.
asianum—
were identified from
T. hemsleyanum
using multilocus sequences analysis and phylogenetic analysis.
C. gloeosporioides
was subsequently selected for plant resistance evaluation. Through systematic screening, eight highly resistant (HR) accessions and one highly susceptible (HS) accession of
T. hemsleyanum
were identified. The results demonstrated that antioxidant enzyme activities in the leaves of the HR accession increased significantly more than those in the HS accession within 24 h post‐inoculation (hpi). Moreover, key genes involved in flavonoid biosynthesis and resistance mechanisms were significantly up‐regulated in the HR accession 72 hpi. These findings lay a foundational basis for further research into resistance genes and the development of disease‐resistant
T. hemsleyanum
cultivars.
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