Identification and validation of reference genes for Populus euphratica gene expression analysis during abiotic stresses by quantitative real‐time PCR

Hou‐Ling Wang; Jinhuan Chen; Qianqian Tian; Xinli Xia; Weilun Yin

doi:10.1111/ppl.12206

journal article Jun 02, 2014

Identification and validation of reference genes for Populus euphratica gene expression analysis during abiotic stresses by quantitative real‐time PCR

Hou‐Ling Wang

Jinhuan Chen

Qianqian Tian Xinli Xia

Weilun Yin

Physiologia Plantarum Vol. 152 No. 3 pp. 529-545 · Wiley

View at Publisher Save 10.1111/ppl.12206

Abstract

Populus euphratica is the only arboreal species that is established in the world's largest shifting‐sand desert in China and is well‐adapted to the extreme desert environment, so it is widely considered a model system for researching into abiotic stress resistance of woody plants. However, few P. euphratica reference genes (RGs) have been identified for quantitative real‐time polymerase chain reaction (qRT‐PCR) until now. Validation of suitable RGs is essential for gene expression normalization research. In this study, we screened 16 endogenous candidate RGs in P. euphratica leaves in six abiotic stress treatments, including abscisic acid (ABA), cold, dehydration, drought, short‐duration salt (SS) and long‐duration salt (LS) treatments, each with 6 treatment gradients. After calculation of PCR efficiencies, three different software tools, NormFinder, geNorm and BestKeeper, were employed to analyze the qRT‐PCR data systematically, and the outputs were merged by means of a non‐weighted unsupervised rank aggregation method. The genes selected as optimal for gene expression analysis of the six treatments were RPL17 (ribosomal protein L17) in ABA, EF1α (elongation factor‐1 alpha) in cold, HIS (histone superfamily protein H3) in dehydration, GIIα in drought and SS, and TUB (tubulin) in LS. The expression of 60S (the 60S ribosomal protein) varied the least during all treatments. To illustrate the suitability of these RGs, the relative quantifications of three stress‐inducible genes, PePYL1, PeSCOF‐1 and PeSCL7 were investigated with different RGs. The results, calculated using qBasePlus software, showed that compared with the least‐appropriate RGs, the expression profiles normalized by the recommended RGs were closer to expectations. Our study provided an important RG application guideline for P. euphratica gene expression characterization.

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Metrics

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References

Details

Published: Jun 02, 2014
Vol/Issue: 152(3)
Pages: 529-545
License: View

Authors

H

Hou‐Ling Wang

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology Beijing Forestry University Beijing 100083 China; Key Laboratory for Silviculture and Conservation, College of Forestry Beijing Forestry University Beijing 100083 China

J

Jinhuan Chen

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology Beijing Forestry University Beijing 100083 China

Q

Qianqian Tian

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology Beijing Forestry University Beijing 100083 China

X

Xinli Xia

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology Beijing Forestry University Beijing 100083 China

W

Weilun Yin

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology Beijing Forestry University Beijing 100083 China; Key Laboratory for Silviculture and Conservation, College of Forestry Beijing Forestry University Beijing 100083 China

Cite This Article

Hou‐Ling Wang, Jinhuan Chen, Qianqian Tian, et al. (2014). Identification and validation of reference genes for Populus euphratica gene expression analysis during abiotic stresses by quantitative real‐time PCR. Physiologia Plantarum, 152(3), 529-545. https://doi.org/10.1111/ppl.12206

Identification and validation of reference genes for Populus euphratica gene expression analysis during abiotic stresses by quantitative real‐time PCR

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