Bacillus subtilis promotes plant phosphorus (P) acquisition through P solubilization and stimulation of root and root hair growth

Camilla Niketa Gadomska Jensen; Janet Ka Yan Pang; Michele Gottardi; Stjepan Krešimir Kračun; Birgit Albrecht Svendsen; Kristian Fog Nielsen; Ákos T. Kovács; Lars Moelbak; Lorenzo Fimognari; Søren Husted; Alexander Schulz

doi:10.1111/ppl.14338

journal article Open Access May 01, 2024

Bacillus subtilis promotes plant phosphorus (P) acquisition through P solubilization and stimulation of root and root hair growth

Camilla Niketa Gadomska Jensen

Janet Ka Yan Pang Michele Gottardi Stjepan Krešimir Kračun

Birgit Albrecht Svendsen Kristian Fog Nielsen

Ákos T. Kovács

Lars Moelbak Lorenzo Fimognari

Søren Husted

Alexander Schulz

Physiologia Plantarum Vol. 176 No. 3 · Wiley

View at Publisher Save 10.1111/ppl.14338

Abstract

AbstractBacteria can be applied as biofertilizers to improve crop growth in phosphorus (P)‐limited conditions. However, their mode of action in a soil environment is still elusive. We used the strain ALC_02 as a case study to elucidate how Bacillus subtilis affects dwarf tomato cultivated in soil‐filled rhizoboxes over time. ALC_02 improved plant P acquisition by increasing the size and P content of P‐limited plants. We assessed three possible mechanisms, namely root growth stimulation, root hair elongation, and solubilization of soil P. ALC_02 produced auxin, and inoculation with ALC_02 promoted root growth. ALC_02 promoted root hair elongation as the earliest observed response and colonized root hairs specifically. Root and root hair growth stimulation was associated with a subsequent increase in plant P content, indicating that a better soil exploration by the root system improved plant P acquisition. Furthermore, ALC_02 affected the plant‐available P content in sterilized soil differently over time and released P from native P pools in the soil. Collectively, ALC_02 exhibited all three mechanisms in a soil environment. To our knowledge, bacterial P biofertilizers have not been reported to colonize and elongate root hairs in the soil so far, and we propose that these traits contribute to the overall effect of ALC_02. The knowledge gained in this research can be applied in the future quest for bacterial P biofertilizers, where we recommend assessing all three parameters, not only root growth and P solubilization, but also root hair elongation. This will ultimately support the development of sustainable agricultural practices.

Topics

No keywords indexed for this article. Browse by subject →

References

62

[1]

10.1038/s41467-020-18326-7

[2]

10.1016/j.micres.2017.03.006

[3]

Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria

Naveen Kumar Arora, Maya Verma

3 Biotech 10.1007/s13205-017-1008-y

[4]

Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of Biostimulants for Sustainable Agriculture

Rachel Backer, J. Stefan Rokem, Gayathri Ilangumaran et al.

Frontiers in Plant Science 10.3389/fpls.2018.01473

[5]

10.4067/s0718‐95162012005000015

[6]

10.1111/nph.17074

[7]

10.1139/m97-015

[8]

10.1007/s00374-012-0737-7

[9]

10.1007/s00203-021-02361-z

[10]

10.1094/mpmi-08-20-0225-cr

[11]

10.1093/aob/mcs231

[12]

10.1104/pp.17.01624

[13]

10.1111/aab.12673

[14]

10.1016/j.jclepro.2022.133110

[15]

10.1016/j.scienta.2015.09.021

[16]

10.3389/fpls.2020.00979

[17]

10.1007/s12275-012-1439-4

[18]

10.1023/a:1004346412006

[19]

10.1016/j.geoderma.2018.11.046

[20]

10.1007/s11104-018-3756-9

[21]

COLORIMETRIC ESTIMATION OF INDOLEACETIC ACID

Solon A. Gordon, Robert P. Weber

Plant Physiology 10.1104/pp.26.1.192

[22]

10.1007/s11104-023-05901-2

[23]

10.3390/agronomy12102539

[24]

10.1016/j.plantsci.2023.111936

[25]

10.1111/jac.12639

[26]

10.5424/sjar/2016144-9714

[27]

10.1094/mpmi-20-2-0207

[28]

Magid J. (2006)

[29]

10.1111/jam.15480

[30]

10.1093/aob/mcaa181

[31]

10.1016/s0003-2670(00)88444-5

[32]

10.1007/s11104-017-3528-y

[33]

10.1016/s0378-1097(98)00555-2

[34]

Olsen S. R. Cole C. V. &Watanabe F. S.(1954).Estimation of available phosphorus in soils by extraction with sodium bicarbonate.USDA Circular No. 939 US Government Printing Office Washington DC.

[35]

10.1101/cshperspect.a001537

[36]

10.1016/j.apsoil.2014.09.012

[37]

10.1016/j.rhisph.2016.08.001

[38]

10.1186/s43141-021-00252-w

[39]

10.1104/pp.114.244541

[40]

10.1016/s0038-0717(00)00010-9

[41]

10.1111/nph.16924

[42]

10.1101/cshperspect.a039933

[43]

Rubæk G. H. (2015)

[44]

10.1007/s11104-019-04308-2

[45]

10.1093/aob/mcab104

[46]

10.1016/j.envexpbot.2011.11.026

[47]

Universal chemical assay for the detection and determination of siderophores

Bernhard Schwyn, J.B. Neilands

Analytical Biochemistry 10.1016/0003-2697(87)90612-9

[48]

10.1093/aob/mcy108

[49]

10.1038/s41598-017-14080-x

[50]

10.1007/s11104-008-9560-1

Showing 50 of 62 references

Metrics

20

Citations

62

References

Details

Published: May 01, 2024
Vol/Issue: 176(3)
License: View

Authors

C

Camilla Niketa Gadomska Jensen

Department of Plant and Environmental Sciences University of Copenhagen Frederiksberg Denmark; Plant Health Innovation Novonesis A/S Taastrup Denmark

J

Janet Ka Yan Pang

Bacterial Interactions and Evolution Group, DTU Bioengineering Technical University of Denmark Kgs. Lyngby Denmark

M

Michele Gottardi

Plant Health Innovation Novonesis A/S Taastrup Denmark

S

Stjepan Krešimir Kračun

R&D Microbial Screening R&D Microbe & Culture Research Novonesis A/S Hørsholm Denmark

B

Birgit Albrecht Svendsen

R&D Microbial Screening R&D Microbe & Culture Research Novonesis A/S Hørsholm Denmark

K

Kristian Fog Nielsen

Biochemical Assays Novonesis A/S Hørsholm Denmark

�

Ákos T. Kovács

Bacterial Interactions and Evolution Group, DTU Bioengineering Technical University of Denmark Kgs. Lyngby Denmark; Institute of Biology Leiden University Leiden the Netherlands

L

Lars Moelbak

Plant Health Innovation Novonesis A/S Taastrup Denmark

L

Lorenzo Fimognari

Plant Health Innovation Novonesis A/S Taastrup Denmark

S

Søren Husted

Department of Plant and Environmental Sciences University of Copenhagen Frederiksberg Denmark

A

Alexander Schulz

Department of Plant and Environmental Sciences University of Copenhagen Frederiksberg Denmark

Cite This Article

Camilla Niketa Gadomska Jensen, Janet Ka Yan Pang, Michele Gottardi, et al. (2024). Bacillus subtilis promotes plant phosphorus (P) acquisition through P solubilization and stimulation of root and root hair growth. Physiologia Plantarum, 176(3). https://doi.org/10.1111/ppl.14338

Bacillus subtilis promotes plant phosphorus (P) acquisition through P solubilization and stimulation of root and root hair growth

You May Also Like