Tryptophan, kynurenine, kynurenic acid concentrations and indoleamine 2,3‐dioxygenase activity in serum and milk of dairy cows with subclinical mastitis caused by coagulase‐negative staphylococci

Mariola Bochniarz; Tomasz Kocki; Roman Dąbrowski; Marek Szczubiał; Władysław Wawron; Waldemar A. Turski

doi:10.1111/rda.13299

journal article Aug 28, 2018

Tryptophan, kynurenine, kynurenic acid concentrations and indoleamine 2,3‐dioxygenase activity in serum and milk of dairy cows with subclinical mastitis caused by coagulase‐negative staphylococci

Mariola Bochniarz

Tomasz Kocki

Roman Dąbrowski

Marek Szczubiał

Władysław Wawron Waldemar A. Turski

Reproduction in Domestic Animals Vol. 53 No. 6 pp. 1491-1497 · Wiley

View at Publisher Save 10.1111/rda.13299

Abstract

ContentsThe aim of the study was to investigate serum and milk concentrations of tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYNA), and indoleamine 2,3‐dioxygenase (IDO) activity in cows suffering from subclinical mastitis caused by coagulase‐negative staphylococci (MSCNS). TRP and kynurenines were determined by high‐performance liquid chromatography (HPLC), and IDO activity was calculated as the KYN/TRP ratio. The blood and milk samples were collected from 40 midlactation Holstein‐Fresian cows from two herds in the Lublin region in Poland. In the milk samples from 20 cows with subclinical mastitis, coagulase‐negative staphylococci were isolated and in the milk obtained from healthy cows growth of microorganisms was not detected. TRP, KYN and KYNA concentrations were significantly lower in milk of cows with MSCNS compared to control animals (4.47 vs. 7.24 µM, 0.14 vs. 0.21 µM, 1.58 vs. 2.18 nM, respectively). There was no statistically significant difference in TRP and KYNA concentrations in serum between the studied animal groups (32.97 vs. 39.29 µM, 31.3 vs. 26.5 nM, respectively). In turn, the level of KYN was lower in the serum (0.81 vs. 1.13 µM) of cows with mastitis compared to healthy ones. No statistically significant differences in IDO activity, both in serum and in milk (25.24 and 27.55, 28.56 and 27.17, respectively) was revealed between the studied groups. These findings may have potential implications for diagnosis of mastitis in cows because reduction of these parameters in milk might be a marker predicting the occurrence of the disease.

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Details

Published: Aug 28, 2018
Vol/Issue: 53(6)
Pages: 1491-1497
License: View

Authors

M

Mariola Bochniarz

Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine University of Life Sciences Lublin Poland

T

Tomasz Kocki

Department of Experimental and Clinical Pharmacology Medical University of Lublin Lublin Poland

R

Roman Dąbrowski

Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine University of Life Sciences Lublin Poland

M

Marek Szczubiał

Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine University of Life Sciences Lublin Poland

W

Władysław Wawron

Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine University of Life Sciences Lublin Poland

W

Waldemar A. Turski

Department of Experimental and Clinical Pharmacology Medical University of Lublin Lublin Poland

Cite This Article

Mariola Bochniarz, Tomasz Kocki, Roman Dąbrowski, et al. (2018). Tryptophan, kynurenine, kynurenic acid concentrations and indoleamine 2,3‐dioxygenase activity in serum and milk of dairy cows with subclinical mastitis caused by coagulase‐negative staphylococci. Reproduction in Domestic Animals, 53(6), 1491-1497. https://doi.org/10.1111/rda.13299

Tryptophan, kynurenine, kynurenic acid concentrations and indoleamine 2,3‐dioxygenase activity in serum and milk of dairy cows with subclinical mastitis caused by coagulase‐negative staphylococci

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