MYBD employed by HY5 increases anthocyanin accumulation via repression of MYBL2 in Arabidopsis

Nguyen Hoai Nguyen; Chan Young Jeong; Geun‐Ho Kang; Sang‐Dong Yoo; Suk‐Whan Hong; Hojoung Lee

doi:10.1111/tpj.13077

journal article Dec 01, 2015

MYBD employed by HY5 increases anthocyanin accumulation via repression of MYBL2 in Arabidopsis

Nguyen Hoai Nguyen Chan Young Jeong Geun‐Ho Kang Sang‐Dong Yoo Suk‐Whan Hong

Hojoung Lee

The Plant Journal Vol. 84 No. 6 pp. 1192-1205 · Wiley

View at Publisher Save 10.1111/tpj.13077

Abstract

SummaryPhotomorphogenesis is an essential program in plant development. This process is effected by the balanced cooperation of many factors under light and dark conditions. In a previous study, we showed that MYB hypocotyl elongation‐related (MYBH) is involved in cell elongation. To expand our understanding of MYBH function, we performed a yeast two‐hybrid assay and identified an MYB‐like Domain transcription factor (MYBD). In this study, we investigated the function of MYBD, which is an MYBH homolog involved in anthocyanin accumulation. MYBD expression increased in response to light or cytokinin, and MYBD enhanced anthocyanin biosynthesis via repression of MYBL2, which encodes a transcription factor that has a negative effect on this process. In addition, MYBD binding in vivo to the MYBL2 promoter and the lower level of histone H3K9 acetylation at the upstream region of MYBL2 in MYBD over‐expressing plants in comparison with wild‐type plants imply that MYBD represses MYBL2 expression via an epigenetic mechanism. HY5 directly binds to the MYBD promoter, which indicates that MYBD acts on HY5‐downstream in light‐ or cytokinin‐triggered signaling pathways, leading to anthocyanin accumulation. Our results suggest that, although MYBD and MYBH are homologs, they act in opposite ways during plant photomorphogenesis, and these functions should be examined in further studies.

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Details

Published: Dec 01, 2015
Vol/Issue: 84(6)
Pages: 1192-1205
License: View

Authors

N

Nguyen Hoai Nguyen

Department of Biosystems and Biotechnology College of Life Sciences and Biotechnology Korea University Anam‐dong 5‐ga Seongbuk‐gu Seoul 136‐713 Korea; Institute of Life Science and Natural Resources Korea University Seoul 136‐713 Korea

C

Chan Young Jeong

Department of Biosystems and Biotechnology College of Life Sciences and Biotechnology Korea University Anam‐dong 5‐ga Seongbuk‐gu Seoul 136‐713 Korea; Institute of Life Science and Natural Resources Korea University Seoul 136‐713 Korea

G

Geun‐Ho Kang

Department of Life Sciences College of Life Sciences and Biotechnology Korea University Anam‐dong 5‐ga Seongbuk‐gu Seoul 136‐713 Korea

S

Sang‐Dong Yoo

Department of Life Sciences College of Life Sciences and Biotechnology Korea University Anam‐dong 5‐ga Seongbuk‐gu Seoul 136‐713 Korea

S

Suk‐Whan Hong

Department of Molecular Biotechnology College of Agriculture and Life Sciences Bioenergy Research Center Chonnam National University Gwangju Korea

H

Hojoung Lee

Department of Biosystems and Biotechnology College of Life Sciences and Biotechnology Korea University Anam‐dong 5‐ga Seongbuk‐gu Seoul 136‐713 Korea; Institute of Life Science and Natural Resources Korea University Seoul 136‐713 Korea

Funding

National Research Foundation of Korea Award: NRF‐2014R1A1A3050272

Cite This Article

Nguyen Hoai Nguyen, Chan Young Jeong, Geun‐Ho Kang, et al. (2015). MYBD employed by HY5 increases anthocyanin accumulation via repression of MYBL2 in Arabidopsis. The Plant Journal, 84(6), 1192-1205. https://doi.org/10.1111/tpj.13077

MYBD employed by HY5 increases anthocyanin accumulation via repression of MYBL2 in Arabidopsis

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