PP7L is essential for MAIL1‐mediated transposable element silencing and primary root growth

Cloe de Luxán‐Hernández; Julia Lohmann; Wiebke Hellmeyer; Senoch Seanpong; Kerstin Wöltje; Zoltan Magyar; Aladár Pettkó‐Szandtner; Thierry Pélissier; Geert De Jaeger; Stefan Hoth; Olivier Mathieu; Magdalena Weingartner

doi:10.1111/tpj.14655

journal article Open Access Jan 25, 2020

PP7L is essential for MAIL1‐mediated transposable element silencing and primary root growth

Cloe de Luxán‐Hernández Julia Lohmann

Wiebke Hellmeyer Senoch Seanpong Kerstin Wöltje Zoltan Magyar Aladár Pettkó‐Szandtner Thierry Pélissier

Geert De Jaeger

Stefan Hoth Olivier Mathieu

Magdalena Weingartner

The Plant Journal Vol. 102 No. 4 pp. 703-717 · Wiley

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Abstract

SummaryThe two paralogous Arabidopsis genes MAINTENANCE OF MERISTEMS (MAIN) and MAINTENANCE OF MERISTEMS LIKE1 (MAIL1) encode a conserved retrotransposon‐related plant mobile domain and are known to be required for silencing of transposable elements (TE) and for primary root development. Loss of function of either MAIN or MAIL1 leads to release of heterochromatic TEs, reduced condensation of pericentromeric heterochromatin, cell death of meristem cells and growth arrest of the primary root soon after germination. Here, we show that they act in one protein complex that also contains the inactive isoform of PROTEIN PHOSPHATASE 7 (PP7), which is named PROTEIN PHOSPHATASE 7‐LIKE (PP7L). PP7L was previously shown to be important for chloroplast biogenesis and efficient chloroplast protein synthesis. We show that loss of PP7L function leads to the same root growth phenotype as loss of MAIL1 or MAIN. In addition, pp7l mutants show similar silencing defects. Double mutant analyses confirmed that the three proteins act in the same molecular pathway. The primary root growth arrest, which is associated with cell death of stem cells and their daughter cells, is a consequence of genome instability. Our data demonstrate so far unrecognized functions of an inactive phosphatase isoform in a protein complex that is essential for silencing of heterochromatic elements and for maintenance of genome stability in dividing cells.

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Details

Published: Jan 25, 2020
Vol/Issue: 102(4)
Pages: 703-717
License: View

Authors

C

Cloe de Luxán‐Hernández

Molecular Plant Physiology Institute for Plant Science and Microbiology Universität Hamburg Hamburg 22609 Germany

J

Julia Lohmann

Molecular Plant Physiology Institute for Plant Science and Microbiology Universität Hamburg Hamburg 22609 Germany

W

Wiebke Hellmeyer

Molecular Plant Physiology Institute for Plant Science and Microbiology Universität Hamburg Hamburg 22609 Germany

S

Senoch Seanpong

Molecular Plant Physiology Institute for Plant Science and Microbiology Universität Hamburg Hamburg 22609 Germany

K

Kerstin Wöltje

Molecular Plant Physiology Institute for Plant Science and Microbiology Universität Hamburg Hamburg 22609 Germany

Z

Zoltan Magyar

Institute of Plant Biology Biological Research Centre Szeged 6726 Hungary

A

Aladár Pettkó‐Szandtner

Institute of Plant Biology Biological Research Centre Szeged 6726 Hungary; Laboratory of Proteomics Research Biological Research Centre Temesvári krt. 62 6726 Szeged Hungary

T

Thierry Pélissier

GReD – CNRS UMR6293 – Inserm U1103 Université Clermont Auvergne UFR de Médecine Clermont‐Ferrand Cedex France

G

Geert De Jaeger

VIB Center for Plant Systems Biology 9052 Gent Belgium; Department of Plant Biotechnology and Bioinformatics Ghent University Technologiepark 71 9052 Gent Belgium

S

Stefan Hoth

Molecular Plant Physiology Institute for Plant Science and Microbiology Universität Hamburg Hamburg 22609 Germany

O

Olivier Mathieu

GReD – CNRS UMR6293 – Inserm U1103 Université Clermont Auvergne UFR de Médecine Clermont‐Ferrand Cedex France

M

Magdalena Weingartner

Molecular Plant Physiology Institute for Plant Science and Microbiology Universität Hamburg Hamburg 22609 Germany

Funding

Deutsche Forschungsgemeinschaft Award: 4506/6‐1

Cite This Article

Cloe de Luxán‐Hernández, Julia Lohmann, Wiebke Hellmeyer, et al. (2020). PP7L is essential for MAIL1‐mediated transposable element silencing and primary root growth. The Plant Journal, 102(4), 703-717. https://doi.org/10.1111/tpj.14655

PP7L is essential for MAIL1‐mediated transposable element silencing and primary root growth

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