Self S‐RNase inhibits ABF–LRX signaling to arrest pollen tube growth to achieve self‐incompatibility in pear

Lei Wu; Xing Liu; Ming‐Yue Zhang; Kai‐Jie Qi; Xiaosen Jiang; Jia‐Long Yao; Shao‐Ling Zhang; Chao Gu

doi:10.1111/tpj.16072

journal article Jan 09, 2023

Self S‐RNase inhibits ABF–LRX signaling to arrest pollen tube growth to achieve self‐incompatibility in pear

Lei Wu

Xing Liu

Ming‐Yue Zhang Kai‐Jie Qi Xiaosen Jiang Jia‐Long Yao

Shao‐Ling Zhang

Chao Gu

The Plant Journal Vol. 113 No. 3 pp. 595-609 · Wiley

View at Publisher Save 10.1111/tpj.16072

Abstract

SUMMARYGametophytic self‐incompatibility (GSI) has been widely studied in flowering plants, but studies of the mechanisms underlying pollen tube growth arrest by self S‐RNase in GSI species are limited. In the present study, two leucine‐rich repeat extensin genes in pear (Pyrus bretschneideri), PbLRXA2.1 and PbLRXA2.2, were identified based on transcriptome and quantitative real‐time PCR analyses. The expression levels of these two LRX genes were significantly higher in the pollen grains and pollen tubes of the self‐compatible cultivar ‘Jinzhui’ (harboring a spontaneous bud mutation) than in those of the self‐incompatible cultivar ‘Yali’. Both PbLRXA2.1 and PbLRXA2.2 stimulated pollen tube growth and attenuated the inhibitory effects of self S‐RNase on pollen tube growth by stabilizing the actin cytoskeleton and enhancing cell wall integrity. These results indicate that abnormal expression of PbLRXA2.1 and PbLRXA2.2 is involved in the loss of self‐incompatibility in ‘Jinzhui’. The PbLRXA2.1 and PbLRXA2.2 promoters were directly bound by the ABRE‐binding factor PbABF.D.2. Knockdown of PbABF.D.2 decreased PbLRXA2.1 and PbLRXA2.2 expression and inhibited pollen tube growth. Notably, the expression of PbLRXA2.1, PbLRXA2.2, and PbABF.D.2 was repressed by self S‐RNase, suggesting that self S‐RNase can arrest pollen tube growth by restricting the PbABF.D.2–PbLRXA2.1/PbLRXA2.2 signal cascade. These results provide novel insight into pollen tube growth arrest by self S‐RNase.

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References

Details

Published: Jan 09, 2023
Vol/Issue: 113(3)
Pages: 595-609
License: View

Authors

L

Lei Wu

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

X

Xing Liu

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

M

Ming‐Yue Zhang

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

K

Kai‐Jie Qi

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

X

Xiaosen Jiang

College of Life Sciences Nanjing Agricultural University Nanjing Jiangsu 210095 China

J

Jia‐Long Yao

The New Zealand Institute for Plant and Food Research Limited Private Bag 92169 Auckland 1142 New Zealand

S

Shao‐Ling Zhang

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

C

Chao Gu

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

Funding

National Natural Science Foundation of China Award: 31672118

National Key Research and Development Program of China Award: 2018YFD1000107

Earmarked Fund for China Agriculture Research System Award: CARS‐28

Cite This Article

Lei Wu, Xing Liu, Ming‐Yue Zhang, et al. (2023). Self S‐RNase inhibits ABF–LRX signaling to arrest pollen tube growth to achieve self‐incompatibility in pear. The Plant Journal, 113(3), 595-609. https://doi.org/10.1111/tpj.16072

Self S‐RNase inhibits ABF–LRX signaling to arrest pollen tube growth to achieve self‐incompatibility in pear

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