Transcellular vesicular transport in epithelial and endothelial cells: Challenges and opportunities

Karen Y. Y. Fung; Gregory D. Fairn; Warren L. Lee

doi:10.1111/tra.12533

journal article Nov 21, 2017

Transcellular vesicular transport in epithelial and endothelial cells: Challenges and opportunities

Karen Y. Y. Fung Gregory D. Fairn

Warren L. Lee

Traffic Vol. 19 No. 1 pp. 5-18 · Wiley

View at Publisher Save 10.1111/tra.12533

Abstract

Vesicle‐mediated transcellular transport or simply “transcytosis” is a cellular process used to shuttle macromolecules such as lipoproteins, antibodies, and albumin from one surface of a polarized cell to the other. This mechanism is in contrast to the transit of small molecules such as anions, cations and amino acids that occur via uptake, diffusion through the cytosol and release and is also distinct from paracellular leak between cells. Importantly, transcytosis has evolved as a process to selectively move macromolecules between 2 neighboring yet unique microenvironments within a multicellular organism. Examples include the movement of lipoproteins out of the circulatory system and into tissues and the delivery of immunoglobulins to mucosal surfaces. Regardless of whether the transport is conducted by endothelial or epithelial cells, the process often involves receptor‐mediated uptake of a ligand into an endocytic vesicle, regulated transit of the carrier through the cytoplasm and release of the cargo via an exocytic event. While transcytosis has been examined in detail in epithelial cells, for both historical and technical reasons, the process is less understood in endothelial cells. Here, we spotlight aspects of epithelial transcytosis including recent findings and review the comparative dearth of knowledge regarding the process in endothelial cells highlighting the opportunity for further study.

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Details

Published: Nov 21, 2017
Vol/Issue: 19(1)
Pages: 5-18
License: View

Authors

K

Karen Y. Y. Fung

Keenan Research Centre for Biomedical Science St. Michael’s Hospital Toronto Ontario Canada; Department of Biochemistry University of Toronto Toronto Ontario Canada

G

Gregory D. Fairn

Keenan Research Centre for Biomedical Science St. Michael’s Hospital Toronto Ontario Canada; Department of Biochemistry University of Toronto Toronto Ontario Canada; Department of Surgery & Institute of Medical Science University of Toronto Toronto Ontario Canada; Institute for Biomedical Engineering and Science Technology (iBEST) Ryerson University and St Michael's Hospital Toronto Ontario Canada

W

Warren L. Lee

Keenan Research Centre for Biomedical Science St. Michael’s Hospital Toronto Ontario Canada; Department of Biochemistry University of Toronto Toronto Ontario Canada; Institute for Biomedical Engineering and Science Technology (iBEST) Ryerson University and St Michael's Hospital Toronto Ontario Canada; Departments of Medicine, Laboratory Medicine and Pathobiology,& Institute of Medical Science University of Toronto Toronto Ontario Canada

Funding

Natural Sciences and Engineering Research Council of Canada

Heart and Stroke Foundation of Canada

Cite This Article

Karen Y. Y. Fung, Gregory D. Fairn, Warren L. Lee (2017). Transcellular vesicular transport in epithelial and endothelial cells: Challenges and opportunities. Traffic, 19(1), 5-18. https://doi.org/10.1111/tra.12533

Transcellular vesicular transport in epithelial and endothelial cells: Challenges and opportunities

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