Secretion of a low‐molecular‐weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells

Andrew Samy; Noriko Yamano‐Adachi; Yuichi Koga; Takeshi Omasa

doi:10.1111/tra.12818

journal article Open Access Sep 27, 2021

Secretion of a low‐molecular‐weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells

Andrew Samy

Noriko Yamano‐Adachi

Yuichi Koga

Takeshi Omasa

Traffic Vol. 22 No. 12 pp. 425-438 · Wiley

View at Publisher Save 10.1111/tra.12818

Abstract

AbstractGRP94 (glucose‐regulated protein 94) is a well‐studied chaperone with a lysine, aspartic acid, glutamic acid and leucine (KDEL) motif at its C‐terminal, which is responsible for GRP94 localization in the endoplasmic reticulum (ER). GRP94 is upregulated during ER stress to help fold unfolded proteins or direct proteins to ER‐associated degradation. In a previous study, engineered GRP94 without the KDEL motif stimulated a powerful immune response in vaccine cells. In this report, we show that endogenous GRP94 is naturally secreted into the medium in a truncated form that lacks the KDEL motif in Chinese hamster ovary cells. The secretion of the truncated form of GRP94 was stimulated by the induction of ER stress. These truncations prevent GRP94 recognition by KDEL receptors and retention inside the cell. This study sheds light on a potential trafficking phenomenon during the unfolded protein response that may help understand the functional role of GRP94 as a trafficking molecule.

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References

Details

Published: Sep 27, 2021
Vol/Issue: 22(12)
Pages: 425-438
License: View

Authors

A

Andrew Samy

Department of Biotechnology Graduate School of Engineering, Osaka University Suita Japan

N

Noriko Yamano‐Adachi

Department of Biotechnology Graduate School of Engineering, Osaka University Suita Japan; Industrial Biotechnology Initiative Division, Institute for Open and Transdisciplinary Research Initiatives Osaka University Suita Japan

Y

Yuichi Koga

Department of Biotechnology Graduate School of Engineering, Osaka University Suita Japan; Industrial Biotechnology Initiative Division, Institute for Open and Transdisciplinary Research Initiatives Osaka University Suita Japan

T

Takeshi Omasa

Department of Biotechnology Graduate School of Engineering, Osaka University Suita Japan; Industrial Biotechnology Initiative Division, Institute for Open and Transdisciplinary Research Initiatives Osaka University Suita Japan

Funding

Ministry of Education, Culture, Sports, Science and Technology Award: JP17H06157

Japan Agency for Medical Research and Development Award: JP20ae0101066

Cite This Article

Andrew Samy, Noriko Yamano‐Adachi, Yuichi Koga, et al. (2021). Secretion of a low‐molecular‐weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells. Traffic, 22(12), 425-438. https://doi.org/10.1111/tra.12818

Secretion of a low‐molecular‐weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells

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