Seroprevalence of hepatitis E virus (HEV) and detection of HEV RNA with a transcription‐mediated amplification assay in blood donors from Catalonia (Spain)

Silvia Sauleda; Edgar Ong; Marta Bes; Alanna Janssen; Robin Cory; Maria Babizki; Tim Shin; Andre Lindquist; Anh Hoang; Lee Vang; Maria Piron; Natàlia Casamitjana; Marco Koppelman; Lisa Danzig; Jeffrey M. Linnen

doi:10.1111/trf.12929

journal article Nov 18, 2014

Seroprevalence of hepatitis E virus (HEV) and detection of HEV RNA with a transcription‐mediated amplification assay in blood donors from Catalonia (Spain)

Silvia Sauleda Edgar Ong Marta Bes Alanna Janssen Robin Cory Maria Babizki Tim Shin Andre Lindquist Anh Hoang Lee Vang Maria Piron Natàlia Casamitjana Marco Koppelman Lisa Danzig Jeffrey M. Linnen

Transfusion Vol. 55 No. 5 pp. 972-979 · Wiley

View at Publisher Save 10.1111/trf.12929

Abstract

BackgroundHepatitis E virus (HEV) is an emerging threat to the safety of blood transfusion. The aim of this study was to determine HEV immunoglobulin (Ig)G and RNA prevalence in Catalan blood donors.Study Design and MethodsNearly 10,000 samples were collected from anonymized, unpaid donors at the Banc de Sang i Teixits (Barcelona, Spain) from June to December 2013. For the serology study, a subset of 1082 donations was tested in parallel for HEV IgG using Wantai and Mikrogen enzyme‐linked immunosorbent assay tests. Samples were tested individually (individual‐donation nucleic acid test [ID‐NAT]) for HEV RNA using the Procleix HEV assay (95% limit of detection 7.9 IU/mL). Procleix repeat‐reactive donations were confirmed by an in‐house real‐time polymerase chain reaction (PCR) test.ResultsThe prevalences of IgG anti‐HEV in Catalan blood donors were 19.96% (Wantai assay) and 10.72% (Mikrogen assay). Screening of 9998 samples with the Procleix HEV assay yielded three real‐time PCR‐confirmed and IgM and IgG anti‐HEV–positive donations with viral loads of 250, 564, and 2755 IU/mL. The donation with highest viral load was genotype 3f. HEV RNA positivity rate was one per 3333 donations (0.03%; 95% confidence interval, 0.01%‐0.09%).ConclusionThe Procleix HEV ID‐NAT screening system has provided evidence of HEV RNA presence in Catalan blood donors. Further data are needed to assess the impact of HEV infection in at‐risk patients to design the best strategy to increase blood safety.

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Details

Published: Nov 18, 2014
Vol/Issue: 55(5)
Pages: 972-979
License: View

Authors

S

Silvia Sauleda

Transfusion Safety Laboratory Banc de Sang i Teixits Institut Català de la Salut Barcelona Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd) Barcelona Spain

E

Edgar Ong

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

M

Marta Bes

Transfusion Safety Laboratory Banc de Sang i Teixits Institut Català de la Salut Barcelona Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd) Barcelona Spain

A

Alanna Janssen

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

R

Robin Cory

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

M

Maria Babizki

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

T

Tim Shin

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

A

Andre Lindquist

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

A

Anh Hoang

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

L

Lee Vang

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

M

Maria Piron

Transfusion Safety Laboratory Banc de Sang i Teixits Institut Català de la Salut Barcelona Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd) Barcelona Spain

N

Natàlia Casamitjana

Transfusion Safety Laboratory Banc de Sang i Teixits Institut Català de la Salut Barcelona Spain

M

Marco Koppelman

Sanquin Diagnostics National Screening Laboratory of Sanquin (NSS) Amsterdam the Netherlands

L

Lisa Danzig

Grifols (formerly Novartis Diagnostics) Emeryville California

J

Jeffrey M. Linnen

Hologic, Inc. (formerly Gen‐Probe Incorporated) San Diego California

Cite This Article

Silvia Sauleda, Edgar Ong, Marta Bes, et al. (2014). Seroprevalence of hepatitis E virus (HEV) and detection of HEV RNA with a transcription‐mediated amplification assay in blood donors from Catalonia (Spain). Transfusion, 55(5), 972-979. https://doi.org/10.1111/trf.12929

Seroprevalence of hepatitis E virus (HEV) and detection of HEV RNA with a transcription‐mediated amplification assay in blood donors from Catalonia (Spain)

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