Electrical resistance across the blood‐brain barrier in anaesthetized rats: a developmental study.

A M Butt; H C Jones; N J Abbott

doi:10.1113/jphysiol.1990.sp018243

journal article Oct 01, 1990

Electrical resistance across the blood‐brain barrier in anaesthetized rats: a developmental study.

A M Butt H C Jones N J Abbott

The Journal of Physiology Vol. 429 No. 1 pp. 47-62 · Wiley

View at Publisher Save 10.1113/jphysiol.1990.sp018243

Abstract

1. Ion permeability of the blood‐brain barrier was studied by in situ measurement of transendothelial electrical resistance in anaesthetized rats aged between 17 days gestation and 33 days after birth, and by electron microscopic examination of lanthanum permeability in fetal and neonatal rats aged up to 10 days old. 2. The blood‐brain barrier in 17‐ to 20‐day fetuses had a resistance of 310 omega cm2 but was impermeable to lanthanum, and therefore had properties intermediate between leaky and tight epithelia. 3. From 21 days gestation, the resistance was 1128 omega cm2, indicating a tight blood‐brain barrier and low ion permeability. There was little further change in barrier resistance after birth, and in 28‐ to 33‐day rats, when the brain barrier systems are mature in other ways, vessels had a mean resistance of 1462 omega cm2. 4. In the tight blood‐brain barrier, arterial vessels had a significantly higher resistance than venous vessels, 1490 and 918 omega cm2 respectively. In vessels less than 50 microns diameter and within the normal 60 min experimental period, there was no significant variation in vessel resistance. 5. Hyperosmotic shock caused a rapid decay in resistance (maximal within 5 min), and after disruption of the blood‐brain barrier, vessel resistance was 100‐300 omega cm2 in both arterial and venous vessels, and the effect was reversible. After the application of metabolic poisons (NaCN plus iodoacetate) and low temperature there was a similarly low electrical resistance. 6. It is concluded that the increase in electrical resistance at birth indicates a decrease in paracellular ion permeability at the blood‐brain barrier and is required for effective brain interstitial fluid ion regulation.

Topics

No keywords indexed for this article. Browse by subject →

Cited By

654

Multiple sclerosis: etiology in the context of neurovascular unit and immune system involvement and advancements with in vitro blood–brain barrier models

Aya A. El-Taibany, Parichehr Heydarian · 2025

Frontiers in Immunology

Homeostasis and dyshomeostasis of the retina

Chang-Jun Zhang, Zi-Bing Jin · 2023

Current Medicine

Cerebrospinal fluid production by the choroid plexus: a century of barrier research revisited

Nanna MacAulay, Richard F. Keep · 2022

Fluids and Barriers of the CNS

Organ‐On‐A‐Chip Models of the Blood–Brain Barrier: Recent Advances and Future Prospects

Satoru Kawakita, Kalpana Mandal · 2022

Small

Unique structural features of claudin‐5 and claudin‐15 lead to functionally distinct tight junction strand architecture

Nandhini Rajagopal, Shikha Nangia · 2022

Annals of the New York Academy of S...

Neuronal and glial regulation of CNS angiogenesis and barriergenesis

Saptarshi Biswas, Azzurra Cottarelli · 2020

Development

Role of Delayed Neuroglial Activation in Impaired Cerebral Blood Flow Restoration Following Comorbid Injury

Zareen Amtul, Jasmine Randhawa · 2019

Cellular and Molecular Neurobiology

The Translational Significance of the Neurovascular Unit

Heather L. McConnell, Cymon N. Kersch · 2017

Journal of Biological Chemistry

Evaluating the involvement of cerebral microvascular endothelial Na+/K+-ATPase and Na+-K+-2Cl– co-transporter in electrolyte fluxes in an in vitro blood–brain barrier model of dehydration

Kasper Lykke, Mette Assentoft · 2017

Journal of Cerebral Blood Flow &amp...

Fatty Acid-Binding Protein 5 Facilitates the Blood–Brain Barrier Transport of Docosahexaenoic Acid

Yijun Pan, Martin J. Scanlon · 2015

Molecular Pharmaceutics

Stepwise Recruitment of Transcellular and Paracellular Pathways Underlies Blood-Brain Barrier Breakdown in Stroke

Daniel Knowland, Ahmet Arac · 2014

Neuron

Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood–brain barrier model for drug permeability studies

Daniela E Eigenmann, Gongda Xue · 2013

Fluids and Barriers of the CNS

The Adaptation of the Cerebral Circulation to Pregnancy: Mechanisms and Consequences

Marilyn J Cipolla · 2013

Journal of Cerebral Blood Flow &amp...

The hCMEC/D3 cell line as a model of the human blood brain barrier

Babette Weksler, Ignacio A Romero · 2013

Fluids and Barriers of the CNS

‘Sealing off the CNS’: cellular and molecular regulation of blood–brain barriergenesis

Julie A Siegenthaler, Fabien Sohet · 2013

Current Opinion in Neurobiology

Pericytes are required for blood–brain barrier integrity during embryogenesis

Richard Daneman, Lu Zhou · 2010

Nature

Astrocyte–endothelial interactions at the blood–brain barrier

N. Joan Abbott, Lars Rönnbäck · 2006

Nature Reviews Neuroscience

Tight junctions of the blood–brain barrier

Hartwig Wolburg, Andrea Lippoldt · 2002

Vascular Pharmacology

Metrics

654

Citations

0

References

Details

Published: Oct 01, 1990
Vol/Issue: 429(1)
Pages: 47-62
License: View

Authors

Cite This Article

A M Butt, H C Jones, N J Abbott (1990). Electrical resistance across the blood‐brain barrier in anaesthetized rats: a developmental study.. The Journal of Physiology, 429(1), 47-62. https://doi.org/10.1113/jphysiol.1990.sp018243

Electrical resistance across the blood‐brain barrier in anaesthetized rats: a developmental study.

You May Also Like