Presynaptic and postsynaptic mechanisms underlie paired pulse depression at single GABAergic boutons in rat collicular cultures

Sergei Kirischuk; John D. Clements; Rosemarie Grantyn

doi:10.1113/jphysiol.2002.021576

journal article Aug 01, 2002

Presynaptic and postsynaptic mechanisms underlie paired pulse depression at single GABAergic boutons in rat collicular cultures

Sergei Kirischuk John D. Clements Rosemarie Grantyn

The Journal of Physiology Vol. 543 No. 1 pp. 99-116 · Wiley

View at Publisher Save 10.1113/jphysiol.2002.021576

Abstract

Paired pulse depression (PPD) is a common form of short‐term synaptic plasticity. The aim of this study was to characterise PPD at the level of a single inhibitory bouton. Low‐density collicular cultures were loaded with the Ca2+ indicator Oregon Green‐1, active boutons were stained with RH414, and action potentials were blocked with TTX. Evoked IPSCs (eIPSCs) and presynaptic Ca2+ transients were recorded in response to direct presynaptic depolarisation of an individual bouton. The single bouton eIPSCs had a low failure rate (< 0.1), large average quantal content (3‐6) and slow decay (τ1= 15 ms, τ2= 81 ms). The PPD of eIPSCs had two distinct components: PPDfast and PPDslow (τ= 86 ms and 2 s). PPDslow showed no dependence on extracellular Ca2+ concentration, or on the first eIPSC's failure rate or amplitude. Most probably, it reflects a release‐independent inhibition of exocytosis. PPDfast was only observed in normal or elevated Ca2+. It decreased with the failure rate and increased with the amplitude of the first eIPSC. It coincided with paired pulse depression of the presynaptic Ca2+ transients (τ= 120 ms). The decay of the latter was accelerated by EGTA, which also reduced PPDfast. Therefore, a suppressive effect of residual presynaptic Ca2+ on subsequent Ca2+ influx is considered the most likely cause of PPDfast. PPDfast may also have a postsynaptic component, because exposure to a low‐affinity GABAA receptor antagonist (TPMPA; 300 μM) counteracted PPDfast, and asynchronous IPSC amplitudes were depressed for a short interval following an eIPSC. Thus, at these synapses, PPD is produced by at least two release‐independent presynaptic mechanisms and one release‐dependent postsynaptic mechanism.

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Details

Published: Aug 01, 2002
Vol/Issue: 543(1)
Pages: 99-116
License: View

Authors

Johannes Mueller Institute for Physiology, Medical Faculty (Charité) of the Humboldt University Berlin, D-10117 Berlin, Germany

Cite This Article

Sergei Kirischuk, John D. Clements, Rosemarie Grantyn (2002). Presynaptic and postsynaptic mechanisms underlie paired pulse depression at single GABAergic boutons in rat collicular cultures. The Journal of Physiology, 543(1), 99-116. https://doi.org/10.1113/jphysiol.2002.021576

Presynaptic and postsynaptic mechanisms underlie paired pulse depression at single GABAergic boutons in rat collicular cultures

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