Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer

Fei Wang; Jie Long; Liang Li; Zi-Xin Wu; Tian-Tian Da; Xiao-Qing Wang; Chuan Huang; Yi-Hua Jiang; Xue-Qing Yao; Hai-Qing Ma; Zhe-Xiong Lian; Zhi-Bin Zhao; Jie Cao

doi:10.1126/sciadv.adf5464

journal article Jun 16, 2023

Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer

Fei Wang

Jie Long

Liang Li

Zi-Xin Wu Tian-Tian Da Xiao-Qing Wang Chuan Huang

Yi-Hua Jiang Xue-Qing Yao Hai-Qing Ma

Zhe-Xiong Lian

Zhi-Bin Zhao

Jie Cao

Science Advances Vol. 9 No. 24 · American Association for the Advancement of Science (AAAS)

View at Publisher Save 10.1126/sciadv.adf5464

Abstract

In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45
−
nonimmune cells and 196,473 CD45
+
immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors.
F3
+
fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However,
MCAM
+
fibroblasts enriched in liver metastatic tumors might promote generation of CD8_CXCL13 cells through Notch signaling. In summary, we extensively analyzed the transcriptional differences of cell atlas between primary and liver metastatic tumors of CRC by single-cell and spatial transcriptome RNA sequencing, providing different dimensions of the development of liver metastasis in CRC.

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References

Details

Published: Jun 16, 2023
Vol/Issue: 9(24)

Authors

F

Fei Wang

Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.; Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.

J

Jie Long

Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.

L

Liang Li

Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.

Z

Zi-Xin Wu

Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510080, China.

T

Tian-Tian Da

School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, China.

X

Xiao-Qing Wang

School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, China.

C

Chuan Huang

School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, China.

Y

Yi-Hua Jiang

Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.

X

Xue-Qing Yao

Department of General Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.

H

Hai-Qing Ma

Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.

Z

Zhe-Xiong Lian

Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.; Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.

Z

Zhi-Bin Zhao

Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.

J

Jie Cao

Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510080, China.

Cite This Article

Fei Wang, Jie Long, Liang Li, et al. (2023). Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer. Science Advances, 9(24). https://doi.org/10.1126/sciadv.adf5464

Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer

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