Blood-derived APLP1
            +
            extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases

Yuri Choi; Jae Hyun Park; Ala Jo; Chul-Woo Lim; Ji-Min Park; Jin Woo Hwang; Kang Soo Lee; Young-Sang Kim; Hakho Lee; Jisook Moon

doi:10.1126/sciadv.ado6894

journal article Jan 03, 2025

Blood-derived APLP1 + extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases

Yuri Choi

Jae Hyun Park

Ala Jo Chul-Woo Lim Ji-Min Park

Jin Woo Hwang Kang Soo Lee

Young-Sang Kim

Hakho Lee

Jisook Moon

Science Advances Vol. 11 No. 1 · American Association for the Advancement of Science (AAAS)

View at Publisher Save 10.1126/sciadv.ado6894

Abstract

The early detection of neurodegenerative diseases necessitates the identification of specific brain-derived biomolecules in peripheral blood. In this context, our investigation delineates the role of amyloid precursor-like protein 1 (APLP1)—a protein predominantly localized in oligodendrocytes and neurons—as a previously unidentified biomarker in extracellular vesicles (EVs). Through rigorous analysis, APLP1
+
EVs from human sera were unequivocally determined to be of cerebral origin. This assertion was corroborated by distinctive small RNA expression patterns of APLP1
+
EVs. The miRNAs’ putative targets within these EVs manifested pronounced expression in the brain, fortifying their neurospecific provenance. We subjected our findings to stringent validation using Thy-1 GFP M line mice, transgenic models wherein GFP expression is confined to hippocampal neurons. An amalgamation of these results with an exhaustive data analysis accentuates the potential of APLP1
+
EVs as cerebrally originated biomarkers. Synthesizing our findings, APLP1
+
EVs are postulated not merely as diagnostic markers but as seminal entities shaping the future trajectory of neurodegenerative disease diagnostics.

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Published: Jan 03, 2025
Vol/Issue: 11(1)

Authors

Y

Yuri Choi

Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea.

J

Jae Hyun Park

Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea.

A

Ala Jo

Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

C

Chul-Woo Lim

Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea.

J

Ji-Min Park

Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea.

J

Jin Woo Hwang

Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea.

K

Kang Soo Lee

Department of Psychiatry, CHA Bundang Medical Center, CHA University College of Medicine, Gyeonggi-do 13496, Republic of Korea.

Y

Young-Sang Kim

Department of Family Medicine, CHA Bundang Medical Center, CHA University College of Medicine, Gyeonggi-do 13496, Republic of Korea.

H

Hakho Lee

Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

J

Jisook Moon

Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea.

Cite This Article

Yuri Choi, Jae Hyun Park, Ala Jo, et al. (2025). Blood-derived APLP1 + extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases. Science Advances, 11(1). https://doi.org/10.1126/sciadv.ado6894

Blood-derived APLP1 + extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases

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