An LRR Receptor Kinase Involved in Perception of a Peptide Plant Hormone, Phytosulfokine

Yoshikatsu Matsubayashi; Mari Ogawa; Akiko Morita; Youji Sakagami

doi:10.1126/science.1069607

journal article May 24, 2002

An LRR Receptor Kinase Involved in Perception of a Peptide Plant Hormone, Phytosulfokine

Yoshikatsu Matsubayashi Mari Ogawa Akiko Morita Youji Sakagami

Science Vol. 296 No. 5572 pp. 1470-1472 · American Association for the Advancement of Science (AAAS)

View at Publisher Save 10.1126/science.1069607

Abstract

The sulfated peptide phytosulfokine (PSK) is an intercellular signal that plays a key role in cellular dedifferentiation and proliferation in plants. Using ligand-based affinity chromatography, we purified a 120-kilodalton membrane protein, specifically interacting with PSK, from carrot microsomal fractions. The corresponding complementary DNA encodes a 1021–amino acid receptor kinase that contains extracellular leucine-rich repeats, a single transmembrane domain, and a cytoplasmic kinase domain. Overexpression of this receptor kinase in carrot cells caused enhanced callus growth in response to PSK and a substantial increase in the number of tritium-labeled PSK binding sites, suggesting that PSK and this receptor kinase act as a ligand-receptor pair.

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We thank H. Kamada and S. Satoh for providing carrot NC cells. Supported by a Grant-in-Aid for Center of Excellence Research (13CE2005) and Scientific Research (12460148 and 13760087). Supporting Online Material www.sciencemag.org/cgi/content/full/296/5572/1470/DC1 Materials and Methods SFigs. 1 to S4 Table S1 References

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Details

Published: May 24, 2002
Vol/Issue: 296(5572)
Pages: 1470-1472

Authors

Y

Yoshikatsu Matsubayashi

Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.

M

Mari Ogawa

Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.

A

Akiko Morita

Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.

Y

Youji Sakagami

Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.

Cite This Article

Yoshikatsu Matsubayashi, Mari Ogawa, Akiko Morita, et al. (2002). An LRR Receptor Kinase Involved in Perception of a Peptide Plant Hormone, Phytosulfokine. Science, 296(5572), 1470-1472. https://doi.org/10.1126/science.1069607

An LRR Receptor Kinase Involved in Perception of a Peptide Plant Hormone, Phytosulfokine

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