Oscillatory Expression of the bHLH Factor Hes1 Regulated by a Negative Feedback Loop

Hiromi Hirata; Shigeki Yoshiura; Toshiyuki Ohtsuka; Yasumasa Bessho; Takahiro Harada; Kenichi Yoshikawa; Ryoichiro Kageyama

doi:10.1126/science.1074560

journal article Oct 25, 2002

Oscillatory Expression of the bHLH Factor Hes1 Regulated by a Negative Feedback Loop

Hiromi Hirata

Shigeki Yoshiura Toshiyuki Ohtsuka

Yasumasa Bessho Takahiro Harada Kenichi Yoshikawa Ryoichiro Kageyama

Science Vol. 298 No. 5594 pp. 840-843 · American Association for the Advancement of Science (AAAS)

View at Publisher Save 10.1126/science.1074560

Abstract

Transcription of messenger RNAs (mRNAs) for Notch signaling molecules oscillates with 2-hour cycles, and this oscillation is important for coordinated somite segmentation. However, the molecular mechanism of such oscillation remains to be determined. Here, we show that serum treatment of cultured cells induces cyclic expression of both mRNA and protein of the Notch effector Hes1, a basic helix-loop-helix (bHLH) factor, with 2-hour periodicity. Cycling is cell-autonomous and depends on negative autoregulation of
hes1
transcription and ubiquitin-proteasome–mediated degradation of Hes1 protein. Because Hes1 oscillation can be seen in many cell types, this clock may regulate timing in many biological systems.

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References

Details

Published: Oct 25, 2002
Vol/Issue: 298(5594)
Pages: 840-843

Authors

H

Hiromi Hirata

Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.

S

Shigeki Yoshiura

Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.

T

Toshiyuki Ohtsuka

Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.

Y

Yasumasa Bessho

Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.

T

Takahiro Harada

Department of Physics, Graduate School of Science, Kyoto University and CREST, Kyoto 606-8502, Japan.

K

Kenichi Yoshikawa

Department of Physics, Graduate School of Science, Kyoto University and CREST, Kyoto 606-8502, Japan.

R

Ryoichiro Kageyama

Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.

Cite This Article

Hiromi Hirata, Shigeki Yoshiura, Toshiyuki Ohtsuka, et al. (2002). Oscillatory Expression of the bHLH Factor Hes1 Regulated by a Negative Feedback Loop. Science, 298(5594), 840-843. https://doi.org/10.1126/science.1074560

Oscillatory Expression of the bHLH Factor Hes1 Regulated by a Negative Feedback Loop

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